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Related Concept Videos

Establishment and Histological Analysis of Esophageal Organoids Modeling the Progression from Normal to Cancerous Tissues05:57

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The present protocol describes the establishment and histological analysis of esophageal organoid models that represent different stages of tumor progression. This method enables researchers to study changes in cellular morphology, spatial organization, and molecular marker expression patterns during the transition from normal to cancerous...
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Related Experiment Video

Updated: Jan 19, 2026

Establishment and Histological Analysis of Esophageal Organoids Modeling the Progression from Normal to Cancerous Tissues
05:57

Establishment and Histological Analysis of Esophageal Organoids Modeling the Progression from Normal to Cancerous Tissues

Published on: May 30, 2025

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ESOPHAGEAL CANCERS AND MODEL SYSTEMS.

Anil K Rustgi1

  • 1PHILADELPHIA, PENNSYLVANIA.

Transactions of the American Clinical and Climatological Association
|September 14, 2019
PubMed
Summary

This study investigates gastrointestinal cancer progression, focusing on how normal cells change, tumors interact with their environment, and how cancer spreads. The goal is to improve treatments for metastatic gastrointestinal cancers.

Area of Science:

  • Gastrointestinal oncology
  • Cancer biology
  • Translational research

Background:

  • Gastrointestinal cancers are common worldwide, originating from epithelial cells in organs like the esophagus, stomach, and liver.
  • Late-stage gastrointestinal cancers have poor patient outcomes.
  • Understanding cancer progression is crucial for developing effective therapies.

Purpose of the Study:

  • To elucidate mechanisms of epithelial cell transformation (metaplasia, dysplasia).
  • To investigate tumor cell invasion and interactions with stromal and immune cells.
  • To understand tumor cell dissemination and distant organ colonization (metastatic organotropism).

Main Methods:

  • Development of three-dimensional (3D) cell culture models.
  • Utilizing genetically engineered mouse models (GEMMs).

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Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
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Related Experiment Videos

Last Updated: Jan 19, 2026

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Published on: May 30, 2025

981
Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model
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  • Focusing on esophageal cancer and 3D models for mechanistic studies.

    • Main Results:

    • Mechanisms of normal epithelial cell metaplasia and dysplasia identified.
    • Tumor cell interactions with activated fibroblasts and immune cells elucidated.
    • Processes of tumor cell dissemination and metastatic organotropism deciphered.

    Conclusions:

    • Preclinical models are being developed to understand gastrointestinal cancer progression.
    • The research aims to translate findings into clinical trials for metastatic disease.
    • Improving outcomes for patients with metastatic gastrointestinal cancers is the ultimate goal.