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Value Chain Analysis - Application01:27

Value Chain Analysis - Application

The value chain analysis enables an organization to identify areas of strength and weakness, reduce costs, and create competitive advantages.
Starbucks utilizes direct grower contact and local suppliers for high-quality raw materials (Inbound Logistics). The company operates roasting plants, innovates its menu, and enhances store ambiance for consistency and quality (Operations). Through owned stores, licensed outlets, and partnerships, including a rewards program and a mobile app, Starbucks...
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Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve09:52

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

We describe fluorescence photoactivation methods to analyze the axonal transport of neurofilaments in single myelinated axons of peripheral nerves from transgenic mice that express a photoactivatable neurofilament...
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Human Egg Maturity Assessment and Its Clinical Application08:51

Human Egg Maturity Assessment and Its Clinical Application

We provide an outline of the clinical protocol for non-invasive assessment of human egg maturity using polarized light...
20.1K
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood09:29

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

T cells expressing a CD19-specific chimeric antigen receptor (CAR) are infused as investigational treatment of B-cell malignancies in our first-in-human gene therapy trials. We describe genetic modification of T cells using the Sleeping Beauty (SB) system to introduce CD19-specific CAR and selective propagation on designer CD19+ artificial antigen presenting...
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Fluorescence Photoactivation Imaging of Neurofilament Transport in Myelinated Mouse Axons04:33

Fluorescence Photoactivation Imaging of Neurofilament Transport in Myelinated Mouse Axons

Source: Boyer, N. P. et. al., Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve. J. Vis. Exp. (2020) This video demonstrates the fluorescence photoactivation method to analyze neurofilament transport in myelinated axons of peripheral nerves. By tracking photoactivated green fluorophore-tagged neurofilaments, the technique provides insights into axonal transport in neurodegenerative...
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A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators10:30

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic...
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