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Updated: Jan 19, 2026

Methodology for Accurate Detection of Mitochondrial DNA Methylation
Published on: May 20, 2018
Organic small molecule for detection and photodegradation of mitochondrial DNA mutations
Chien-Hui Su1, Jyun-Wei Chen, Li-Da Chen
1Department of Mechanical Engineering, National Chung-Hsing University, Taichung, Taiwan.
Abstract:
A detection and degradation platform was developed to optically quantify the 6-enolate, 8-keto-dG, an important tautomer of mitochondrial mutated DNA 8-oxo-dG. We first found that 6-enolate, 8-keto-dG offers particular fluorescence emission under the conditions between pH ∼ 7 and ∼11. Thus, a mitochondria-targeting photosensitizer NV-12P was prepared to offer simultaneously photoinduced electron transfer and fluorescence resonance energy transfer (FRET) with 6-enolate, 8-keto-dG. Furthermore, NV-12P can also generate a reactive oxygen species to degrade 6-enolate, 8-keto-dG under irradiation conditions. This is the first publication about optical characterization, concentration detection and photodegradation of 6-enolate, 8-keto-dG, either in biological or in vitro applications.
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