In vitro and in vivo anti-inflammatory activity of a succinoglycan Riclin from Agrobacterium sp. ZCC3656
1Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, China.
Aims:
To purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS-secreting performance and investigate its anti-inflammatory activity using lipopolysaccharide (LPS)-induced macrophage cells in an acute liver injury mouse model.
Methods And Results:
Twelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source. Agrobacterium sp. ZCC3656 exhibited the highest EPS yield (21·1 g l-1 ) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays. The EPS, designated Riclin, was purified by deproteinization using the Sevag method. The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan-like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8. : 1·0 : 0·9 : 1·1 and has an molecular weight of approximately 2·5 × 106 Da. Riclin inhibited TNF-α, IL-1β and IL-6 expression in LPS-stimulated RAW 264.7 macrophage cells in a dose-dependent manner. In addition, Riclin pretreatment increased the survival rate of D-Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF-α, IL-1β and IL-6.
Conclusions:
Agrobacterium sp. ZCC3656 is a highly stable EPS-producing strain. The EPS Riclin from ZCC3656 is a succinoglycan-type polysaccharide that is noncytotoxic and exhibits remarkable anti-inflammatory effects in vivo and in vitro.
Significance And Impact Of The Study:
Succinoglycans are well known for good rheological properties and their physiological interactions with plants. However, their potential activity towards mammals has received little attention. Our study revealed that the succinoglycan Riclin exhibited excellent anti-inflammatory activities and could be considered as a promising reagent in anti-inflammatory treatment.
Related Concept Videos
08:31Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
05:54An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα
06:46Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
01:33Nanoparticle-Mediated Enhanced Delivery of an Anti-Inflammatory Drug in Microglia-Like Cells
08:27Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
08:32Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo