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Photobiomodulation Under Electroencephalographic Controls of Sleep for Stimulation of Lymphatic Removal of Toxins from Mouse Brain07:56

Photobiomodulation Under Electroencephalographic Controls of Sleep for Stimulation of Lymphatic Removal of Toxins from Mouse Brain

This study presents an original and portable tPBM technology under electroencephalographic (EEG) control of deep or non-rapid eye movement (NREM) sleep in non-anesthetized male C57BL/6 mice of different ages to stimulate lymphatic clearance of Aβ from the brain into the peripheral lymphatic system (the deep cervical lymph nodes,...
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Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

A protocol to block lymph flow by surgical suturing of afferent lymphatic vessels is...
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Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated...
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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells08:07

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial...
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Murine Dermal Lymphatic Endothelial Cell Isolation05:52

Murine Dermal Lymphatic Endothelial Cell Isolation

This paper describes a method for magnetic bead-based isolation of murine endothelial cells from dermal lymphatic capillaries. The isolated lymphatic endothelial cells can be used for downstream in vitro experiments and protein expression analysis.
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Development of the Lymphatic System01:15

Development of the Lymphatic System

The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
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