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Related Experiment Video

Updated: Jan 19, 2026

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08:42

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Solid immersion meniscus lens (SIMlens) for open-top light-sheet microscopy.

Lindsey A Barner, Adam K Glaser, Lawrence D True

    Optics Letters
    |September 14, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Open-top light-sheet (OTLS) microscopy enables rapid imaging of large pathology samples. A new solid immersion meniscus lens overcomes previous limitations, allowing multi-resolution imaging with diverse sample clearing protocols.

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    Area of Science:

    • Biomedical optics
    • Microscopy techniques
    • Pathology imaging

    Background:

    • Open-top light-sheet (OTLS) microscopy facilitates rapid volumetric imaging of large pathology specimens.
    • Aberrations arise when light passes through the horizontal sample plate in OTLS microscopy.
    • Existing methods restrict sample movement, refractive index matching, and multi-resolution capabilities.

    Purpose of the Study:

    • To address the challenge of aberrations in OTLS microscopy.
    • To enable multi-resolution imaging for diverse specimen clearing protocols.
    • To introduce a novel optical element for improved OTLS performance.

    Main Methods:

    • Development of a solid immersion meniscus lens.
    • Utilizing the lens as a wavefront-matching element.
    • Testing OTLS microscopy with the new lens and various immersion media.

    Main Results:

    • The solid immersion meniscus lens effectively suppresses aberrations.
    • It enables seamless transition between air and high-index immersion media.
    • Multi-resolution OTLS microscopy is achieved for diverse cleared specimens.

    Conclusions:

    • The solid immersion meniscus lens significantly enhances OTLS microscopy.
    • This innovation overcomes previous technical barriers in large-scale pathology imaging.
    • It broadens the applicability of OTLS for advanced biological sample analysis.