Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

5.4K
This protocol presents an approach to fingerprint and explore multi-dimensional data collected by comprehensive two-dimensional gas chromatography coupled to mass spectrometry. Dedicated pattern recognition algorithms (template matching) are applied to explore the chemical information encrypted in the extra-virgin olive oil volatile fraction (i.e.,...
5.4K
Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography08:22

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

8.1K
Here, we present a method utilizing two-dimensional gas chromatography and nitrogen chemiluminescence detection (GCxGC-NCD) to extensively characterize the different classes of nitrogen-containing compounds in diesel and jet...
8.1K
Gas Chromatography (GC) with Flame-Ionization Detection09:22

Gas Chromatography (GC) with Flame-Ionization Detection

291.3K
Source: Laboratory of Dr. B. Jill Venton - University of Virginia
Gas chromatography (GC) is used to separate and detect small molecular weight compounds in the gas phase. The sample is either a gas or a liquid that is vaporized in the injection port. Typically, the compounds analyzed are less than 1,000 Da, because it is difficult to vaporize larger compounds. GC is popular for environmental monitoring and industrial applications because it is very reliable and can be run nearly continuously....
291.3K
Gas Chromatography: Introduction01:13

Gas Chromatography: Introduction

3.7K
Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...
3.7K
Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry11:44

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

9.8K
A two-dimensional gas chromatography-time-of-flight mass spectrometry method is described for characterization of the aqueous fraction of bio-crude produced from hydrothermal liquefaction of algae. This protocol can also be employed to analyze the aqueous fraction of liquid products from fast pyrolysis, catalytic fast pyrolysis, catalytic deoxygenation and hydro-treating.
9.8K
Gas Chromatography: Overview of Detectors01:13

Gas Chromatography: Overview of Detectors

1.8K
Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Workflow for characterizing volatile oxidation compounds in walnut oil by headspace solid-phase microextraction comprehensive two-dimensional gas chromatography time of flight mass spectrometry coupled with tile-based Fisher ratio analysis and other chemometric tools.

Journal of chromatography. A·2026
Same author

Adapting Tile-Based Discovery Analysis for Comprehensive Two-Dimensional Gas Chromatography with Vacuum Ultraviolet Spectroscopy Data of Gas Oils.

Analytical chemistry·2026
Same author

Investigation of sputum volatiles to classify active tuberculosis: A pilot study.

Tuberculosis (Edinburgh, Scotland)·2026
Same author

Developing 2D mzCompare for single comprehensive two-dimensional chromatography time-of-flight mass spectrometry chromatograms: Substantial resolution enhancement in the context of statistical overlap theory.

Journal of chromatography. A·2026
Same author

Multimodal mass spectrometry imaging for plaque- and region-specific neurolipidomics in Alzheimer's disease mouse models.

Nature communications·2025
Same author

Integrating Model-Based Reconstruction and Deep Learning for Accelerating Mass Spectrometry Imaging.

Analytical chemistry·2025
Same journal

Insights into cellulose/pectin effect in tobermorite/mandarin orange peel composite hydrogels and rehabilitation effect on Cd- and Pb-contaminated slopes.

Journal of chromatography. A·2026
Same journal

Retention prediction in reversed-phase liquid chromatography using XGBoost-based quantitative structure-retention relationships models.

Journal of chromatography. A·2026
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.4K

Dynamic pressure gradient modulation for comprehensive two-dimensional gas chromatography.

Timothy J Trinklein1, Derrick V Gough1, Cable G Warren1

  • 1Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195, United States.

Journal of Chromatography. A
|September 15, 2019
PubMed
Summary
This summary is machine-generated.

A novel dynamic pressure gradient modulation (DPGM) technique enhances comprehensive two-dimensional gas chromatography (GC×GC) separations. This method improves peak capacity and detector response, enabling detailed analysis of complex samples like diesel fuel.

Keywords:
Comprehensive two-dimensional gas chromatographyDifferential flow modulationDynamic pressure gradient modulationHigh peak capacityPulse valve

More Related Videos

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography
08:22

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

Published on: May 15, 2020

8.1K
Gas Chromatography GC with Flame-Ionization Detection
09:22

Gas Chromatography GC with Flame-Ionization Detection

Published on: April 30, 2023

291.3K

Related Experiment Videos

Last Updated: Jan 19, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.4K
Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography
08:22

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

Published on: May 15, 2020

8.1K
Gas Chromatography GC with Flame-Ionization Detection
09:22

Gas Chromatography GC with Flame-Ionization Detection

Published on: April 30, 2023

291.3K

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Separation Science

Background:

  • Comprehensive two-dimensional gas chromatography (GC×GC) is a powerful separation technique.
  • Effective modulation is crucial for GC×GC performance, impacting peak capacity and resolution.
  • Existing modulation techniques face limitations in duty cycle and efficiency.

Purpose of the Study:

  • To introduce and investigate a novel differential flow modulation technique for GC×GC.
  • To demonstrate the capabilities of this new method, termed dynamic pressure gradient modulation (DPGM).
  • To evaluate DPGM's performance in terms of peak capacity, resolution, and detector response.

Main Methods:

  • DPGM was implemented using commercially available components at the junction of the 1D and 2D columns.
  • Carrier gas flow was modulated using auxiliary gas pressure to create cyclic stop-flow and fast-flow conditions.
  • A 90-component mixture and a diesel sample were analyzed to assess performance and applicability.

Main Results:

  • DPGM achieved a 100% duty cycle and full modulation, resulting in narrow 2D peaks (20-180 ms).
  • High 2D peak capacities were achieved (5000 for a 25-min separation, 8640 for a 60-min diesel analysis).
  • Significant detector response enhancement factors (DREF) ranging from 7-87 were observed.

Conclusions:

  • DPGM is a novel and effective modulation technique for GC×GC, utilizing differential flow.
  • The technique provides substantial improvements in separation performance and analyte detectability.
  • DPGM shows promise for the detailed analysis of complex mixtures in various applications.