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Related Concept Videos

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport13:17

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

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Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic...
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Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid04:55

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This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph...
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Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice05:59

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A protocol to block lymph flow by surgical suturing of afferent lymphatic vessels is...
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Lymphatic Vessels and Lymph Transport01:16

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Lymphatic vessels, known as lymphatics, are crucial in transporting lymph from peripheral tissues to our venous system. This process begins with lymph entering through tiny capillaries that branch through tissues. These capillaries have unique features such as larger diameters, thinner walls, and a distinctive one-way valve system formed by overlapping endothelial cells.
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Related Experiment Video

Updated: Jan 19, 2026

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
13:17

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

Published on: March 6, 2015

29.5K

Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.

David C Zawieja1, Sangeetha Thangaswamy2, Wei Wang1

  • 1Department of Medical Physiology, Texas A&M Health Science Center, Temple, TX 76504.

Journal of Immunology (Baltimore, Md. : 1950)
|September 15, 2019
PubMed
Summary
This summary is machine-generated.

Analyzing lymphatic fluid offers precise insights into organ health and disease. This method enables biomarker discovery and serves as an ideal liquid biopsy for physiological and pathological monitoring.

More Related Videos

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
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Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

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Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
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Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid

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Related Experiment Videos

Last Updated: Jan 19, 2026

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
13:17

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

Published on: March 6, 2015

29.5K
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

7.0K
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
04:55

Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid

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Area of Science:

  • Physiology
  • Immunology
  • Biochemistry

Background:

  • Lymphatic fluid directly surrounds organ cells, unlike blood.
  • Lymph composition analysis provides detailed biochemical and cellular health information.
  • Lymphatic fluid is a valuable resource for biomarker discovery.

Purpose of the Study:

  • To describe a protocol for cannulating mouse and rat lymphatic collectors.
  • To enable comprehensive 'omic' sampling and immune cell phenotyping from lymph.
  • To facilitate quantitative immunological studies and monitor organ-specific biochemical changes.

Main Methods:

  • Cannulation of lymphatic collectors in mice and rats.
  • 'Omic' sampling of pre- and postnodal lymph from various anatomical sites.
  • Immune cell phenotyping and molecular injection for trafficking studies.

Main Results:

  • The described protocol allows for detailed analysis of lymphatic fluid.
  • Lymphatic fluid analysis accurately reflects an organ's physiological and pathological state.
  • This method supports biomarker discovery and disease monitoring.

Conclusions:

  • Probing lymphatic fluid provides an accurate snapshot of organ health.
  • Lymphatic fluid analysis is a powerful tool for biomarker discovery.
  • This technique represents an ideal approach for liquid biopsy in organ studies.