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Identification of genes encoding T cell defined tum- antigens
A Van Pel1, E De Plaen, C Lurquin
1Ludwig Institute for Cancer Research, Brussels branch, Brussels, Belgium.
Princess Takamatsu Symposia
|January 1, 1988
Summary
Researchers identified a novel tumor antigen gene, P91A, from immunogenic tumor mutants. This gene encodes a protein recognized by cytolytic T cells (CTL), aiding in the study of tumor-specific transplantation antigens (TSTA).
Area of Science:
- Immunology
- Oncology
- Molecular Biology
Background:
- Tumor mutants (Tum-) express unique tumor antigens, eliciting cytolytic T cell (CTL) responses but not antibody recognition.
- These antigens are crucial for developing tumor-specific transplantation antigens (TSTA) therapies.
- A novel method for cloning genes encoding these transplantation antigens has been developed.
Purpose of the Study:
- To clone genes responsible for tumor-specific transplantation antigens (TSTA).
- To characterize the identified tumor antigen gene P91A.
- To explore the application of the cloning method for isolating new TSTA.
Main Methods:
- Gene transfection of mouse tumor cells.
- Detection of transfectants via CTL stimulation.
- Gene sequencing and data bank comparison.
Main Results:
- Isolation of the tumor antigen gene P91A.
- P91A codes for a 60 kDa protein lacking an N-terminal signal sequence.
- The P91A gene sequence is unrelated to known sequences in data banks.
- Two other unrelated tum- genes were also identified.
Conclusions:
- The developed cloning method is effective for isolating tumor antigen genes.
- The P91A gene represents a novel tumor antigen.
- Further application of this method could identify new mouse and human TSTA.