Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Segmentation of Cortical Grey Matter from T1-Weighted MRI Images06:48

Automated Segmentation of Cortical Grey Matter from T1-Weighted MRI Images

9.4K
This protocol describes the process of applying seven different automated segmentation tools to structural T1-weighted MRI scans to delineate grey matter regions that can be used for the quantification of grey matter volume.
9.4K
Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging09:21

Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging

3.5K
This method uses mass spectrometry imaging (MSI) to understand metabolic processes in S. alba leaves when exposed to xenobiotics. The method allows the spatial localization of compounds of interest and their predicted metabolites within specific, intact...
3.5K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

19.3K
Desorption electrospray ionization mass spectrometry (DESI-MS) is an ambient method by which samples, including biological tissues, can be imaged with minimal sample preparation. By rastering the sample below the ionization probe, this spray-based technique provides sufficient spatial resolution to discern molecular features of interest within tissue...
19.3K
Measuring Grey Matter Differences with Voxel-based Morphometry: The Musical Brain09:17

Measuring Grey Matter Differences with Voxel-based Morphometry: The Musical Brain

18.5K
Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California
Experience shapes the brain. It is well understood that our brains are different as a result of learning. While many experience-related changes manifest themselves at the microscopic level, for example by neurochemical adjustments in the behavior of individual neurons, we may also examine anatomical changes to the structure of the brain at a macroscopic level. One famous example of this kind of...
18.5K
A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology08:36

A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology

8.5K
Here we present methodology for the production of a focal stroke in murine white matter by local injection of an irreversible endothelial nitric oxide synthase (eNOS) inhibitor (L-Nio). Presented are two stereotactic variations, retrograde neuronal tracing, and fresh tissue labeling and dissection that expand the potential applications of this...
8.5K
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows08:16

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

9.4K
This protocol describes a reproducible and reliable method for the sublimation-based preparation of formalin fixed tissue destined for imaging mass...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MAFF regulates ferroptotic sensitivity through iron homeostasis and fatty acid synthesis.

Cell death & disease·2026
Same author

SPARC is associated with tumor nerve stroma interactions and perineural invasion in pancreatic ductal adenocarcinoma.

Scientific reports·2026
Same author

Comprehensive distribution analysis of cyclosporin A on mice tissues applying mass spectrometry imaging.

Analytical and bioanalytical chemistry·2026
Same author

Autophagy-Independent Function of ATG-18 Is Essential for Gonadal Longevity in Caenorhabditis elegans.

Aging cell·2026
Same author

Structure-dependent reactive oxygen species generation by scintillator-free X-ray-activated porphyrins: insights into charge effects and internal conversion quantum yield.

Journal of radiation research·2026
Same author

IgE-dependent anaphylaxis is regulated by sphingolipid binding to activating and inhibitory CD300 family members.

Cell reports·2026

Related Experiment Video

Updated: Jan 19, 2026

Automated Segmentation of Cortical Grey Matter from T1-Weighted MRI Images
06:48

Automated Segmentation of Cortical Grey Matter from T1-Weighted MRI Images

Published on: January 7, 2019

9.4K

Unsupervised machine learning using an imaging mass spectrometry dataset automatically reassembles grey and white

Makoto Nampei1, Makoto Horikawa1,2, Keisuke Ishizu1

  • 1Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, 431-3192, Japan.

Scientific Reports
|September 15, 2019
PubMed
Summary

Imaging mass spectrometry (IMS) visualizes small biomolecule distribution in the brain. This technique successfully mapped lipid patterns and reconstructed brain structures using machine learning, revealing phospholipid localization in the cerebellar cortex.

More Related Videos

Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging
09:21

Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging

Published on: June 15, 2020

3.5K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

19.3K

Related Experiment Videos

Last Updated: Jan 19, 2026

Automated Segmentation of Cortical Grey Matter from T1-Weighted MRI Images
06:48

Automated Segmentation of Cortical Grey Matter from T1-Weighted MRI Images

Published on: January 7, 2019

9.4K
Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging
09:21

Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging

Published on: June 15, 2020

3.5K
Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

19.3K

Area of Science:

  • Neuroscience
  • Analytical Chemistry
  • Biochemistry

Background:

  • Current methods struggle to map small biomolecules like lipids in the brain.
  • Understanding lipid distribution is crucial for brain function and disease research.

Purpose of the Study:

  • To develop and optimize imaging mass spectrometry (IMS) for visualizing small biomolecule distribution.
  • To investigate the spatial distribution of lipids in rat brain tissue.
  • To apply machine learning for brain structure reconstruction based on molecular data.

Main Methods:

  • Utilized solarix XR, a high-resolution MALDI-FT-ICR-MS instrument for molecular detection.
  • Employed imaging mass spectrometry (IMS) to map biomolecule distribution in rat brains.
  • Applied unsupervised machine learning techniques, including principal component analysis (PCA) and hierarchical clustering.

Main Results:

  • Demonstrated differential distribution of phospholipids in neuronal cell bodies and axons.
  • Successfully reconstructed brain structures, including grey and white matter, using PCA without predefined anatomical labels.
  • Classified 10 molecular groups based on distribution patterns, identifying phospholipids as key components in the cerebellar cortex.

Conclusions:

  • IMS is a powerful tool for comprehensive biomolecule distribution analysis in the brain.
  • Machine learning algorithms can effectively reconstruct brain architecture from molecular data.
  • Phospholipids exhibit specific localization patterns within the rat brain, particularly in the cerebellar cortex.