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Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

This protocol standardizes SDS-PAGE analysis for personalized protein corona profiling on nanoparticles, enabling reproducible, scalable, and low-cost detection of cancer-specific signatures. Designed for early diagnosis of pancreatic ductal adenocarcinoma, it offers a practical, REASSURED-aligned alternative to complex proteomic methods in both research and clinical...
291
Quantifying Food Intake in Caenorhabditis elegans by Measuring Bacterial Clearance07:05

Quantifying Food Intake in Caenorhabditis elegans by Measuring Bacterial Clearance

This protocol describes an assay to quantify Caenorhabditis elegans feeding rate based on measuring the clearance of bacteria in liquid...
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Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Here we present a protocol for human Dental Pulp Stem Cells isolation and propagation in order to evaluate the prion protein expression during neuronal differentiation...
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Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves07:12

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

A cyclic pressure bioreactor capable of subjecting heart valve tissue to physiological and pathological pressure conditions has been designed. A LabVIEW program allows users to control pressure magnitude, amplitude and frequency. This device can be used to study the mechanobiology of heart valve tissue or isolated...
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ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

A protocol to detect trichothecenes (mycotoxins of concern for human health) using a newly developed screening method based on a competitive immunochemical method and a final electrochemical detection is demonstrated.
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A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Here, we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for the rapid and highly reproducible obtainment of quantitative data on the speed of cell migration and wound...
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