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Related Concept Videos

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions11:57

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This article describes detailed protocols for ecosystem fabrication of devices (EcoFABs) that enable the studies of plants and plant-microbe interactions in highly controlled laboratory...
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Treatment of Bacteria with Plant Root Exudates to Emulate In Vitro Plant-Microbe Interactions02:49

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Source: Yi, Y., et al. Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates. J. Vis. Exp. (2018)This video demonstrates the preparation and treatment of Bacillus mycoides cultures to study plant-microbe interactions. It outlines the steps from streaking a stock culture and incubating it in liquid medium to exposing the bacteria to plant root exudates under agitation. The process culminates in harvesting and flash-freezing the bacterial cells for...
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A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling11:16

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The described hydroponic cocultivation system supports intact plants with metal mesh screens and cocultivates them with bacteria. Plant tissue, bacteria, and secreted molecules can then be separately harvested for downstream analyses, simultaneously allowing for the molecular responses of both plant hosts and interacting microbes or microbiomes to be...
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Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates08:59

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The goal of the protocol presented here is to study the transcriptomic response of endosphere-isolated Bacillus mycoides to potato root exudates. This method facilitates the identification of important bacterial genes involved in plant-microbe interactions and is in principle applicable to other endophytes and plants, with minor...
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Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts07:16

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Correction: Akhter et al. Silicon-Induced Mitigation of NaCl Stress in Barley (<i>Hordeum vulgare</i> L.), Associated with Enhanced Enzymatic and Non-Enzymatic Antioxidant Activities. <i>Plants</i> 2022<i>, 11</i>, 2379.

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Related Experiment Video

Updated: Jan 19, 2026

Ecosystem Fabrication EcoFAB Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions
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Plant-insect-microbe interaction: A love triangle between enemies in ecosystem.

Ali Noman1, Muhammad Aqeel2, Muhammad Qasim3

  • 1Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China; Department of Botany, Government College University, Faisalabad 38040, Pakistan.

The Science of the Total Environment
|September 15, 2019
PubMed
Summary

Plants engage in complex interactions with microbes and insects, influencing each other through intricate ecological networks. Understanding these multitrophic interactions is crucial for ecological research and reveals plant defense strategies.

Keywords:
EcosystemInteractionsParasitismPlantsSymbiosis

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Area of Science:

  • Ecology
  • Plant-Microbe Interactions
  • Plant-Insect Interactions

Background:

  • Ecosystems feature complex plant interactions with microbes, insects, and other organisms.
  • Previous research often isolates these interactions, overlooking their collective ecological significance.
  • Plants mediate bidirectional interactions between beneficial microbes and insect herbivores, predators, and pollinators.

Purpose of the Study:

  • To integrate ecological and physiological perspectives on plant-microbe-insect interactions.
  • To explore the physiological changes in plants due to microbial pathogenesis and insect herbivory.
  • To highlight the ecological relevance of these multitrophic relationships.

Main Methods:

  • Review of existing literature on plant-microbe and plant-insect interactions.
  • Integration of ecological and physiological data to describe plant responses.
  • Focus on info-chemical changes and food chain dynamics.

Main Results:

  • Plant responses to microbial and insect attacks involve significant morpho-physiological and chemical reprogramming.
  • These changes influence the attraction or repellence of attacking organisms and their natural enemies.
  • Interactions can alter plant info-chemical frameworks, impacting food webs.

Conclusions:

  • Multifaceted plant-microbe-insect interactions are critical in natural ecosystems.
  • Further research into molecular and physiological mechanisms is needed to understand these complex relationships.
  • This review emphasizes the importance of studying microbe-mediated plant-insect interactions and their ecological consequences.