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Glycopeptide Capture for Cell Surface Proteomics
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An overview on enrichment methods for cell surface proteome profiling.

Yanan Li1,2, Hongqiang Qin1, Mingliang Ye1

  • 1CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, 116023, P. R. China.

Journal of Separation Science
|September 15, 2019
PubMed
Summary
This summary is machine-generated.

Analyzing cell surface proteins is crucial for understanding biological processes. This review overviews methods for enriching cell surface proteomes, essential for comprehensive proteomic profiling.

Keywords:
cell surface proteinsenrichment methodsplasma membrane proteinsproteomics

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Area of Science:

  • Proteomics
  • Cell Biology
  • Biochemistry

Background:

  • Cell surface proteins play vital roles in cellular functions.
  • Direct analysis of cell surface proteomes is challenging due to low abundance and high heterogeneity.
  • Selective enrichment is critical for effective proteomic profiling.

Purpose of the Study:

  • To provide a comprehensive overview of cell surface protein enrichment methods.
  • To classify and discuss various techniques for isolating cell surface proteins.
  • To highlight the importance of enrichment for cell surface proteome profiling.

Main Methods:

  • Classification of enrichment methods based on separation mechanisms: physicochemical properties, biomolecular affinity, and chemical covalent coupling.
  • Discussion of metabolic labeling and enzymatic reaction-based isolation techniques.
  • Overview of strategies for enhancing cell surface proteome analysis.

Main Results:

  • Multiple enrichment strategies exist, categorized by their underlying principles.
  • Each method offers distinct advantages for isolating cell surface proteins.
  • The choice of method impacts the comprehensiveness of proteome profiling.

Conclusions:

  • Effective enrichment of cell surface proteins is a prerequisite for in-depth proteomic studies.
  • A variety of advanced techniques are available for selective isolation.
  • This review serves as a guide to selecting appropriate methods for cell surface proteome profiling.