Related Experiment Video
Updated: Jan 19, 2026
SNP Genotyping and GWAS
Published on: April 30, 2023
QTLs identification for nitrogen and phosphorus uptake-related traits using ultra-high density SNP linkage
Youqiang Fu1, Xuhua Zhong1, Junfeng Pan1
1The Rice Research Institute of Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of New Technology for Rice Breeding, Guangzhou 510640, China.
Abstract:
To understand the genetic basis of nitrogen and phosphorus uptake in the cultivated rice, quantitative trait loci (QTL) analysis for 7 nitrogen and phosphorus uptake-related traits including above-ground biomass (AGB), leaf colour value (SPAD) in heading stage, grain nitrogen concentration (GNC), grain nitrogen content of the plant, total nitrogen content (TNC), grain phosphorus concentration, total phosphorus content (TPC) were conducted using SNP markers in a F2 population derived from a cross between GH128 and W6827. A total of 21 QTLs for nitrogen and phosphorus uptake-related traits distributed in 16 regions along 6 chromosomes were detected using a high density genetic map consisting of 1582 bin markers, with QTLs maximum explaining 8.19% of the phenotypic variation. Nine QTLs (42.9% of total QTLs) were detected on chromosome 2. Among them, two QTL clusters including AGB, TNC, TPC and GNC were also detected in the region bin 140 and bin 146 on the chromosome 2. The distance between the two clusters was only 4.1 cM. The presence of QTL clusters has important significance and could be useful in molecular marker assisted breeding. These genomic regions might be deployed for the simultaneous improving the use efficiency of nitrogen and phosphorus in rice breeding.
Related Concept Videos
SNP Genotyping
Soil Nutrient Analysis: Nitrogen, Phosphorus, and Potassium
In this experiment, three soil macronutrients are chemically extracted, combined with color-based reagents, then analyzed using color to determine the nutrient concentration present in the soil sample.
Nitrogen, phosphorus, and potassium are the main components of soil fertilizer. These methods isolate each nutrient from the soil into a solution that can be analyzed using turbidity and color to determine the...
Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
Phosphodiester Linkages
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
03:03Generating an Ultra-Low-Density Neuronal Culture Using a High-Density Neuronal Feeder Layer
Ligand Binding and Linkage