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Related Concept Videos

Alternative RNA Splicing02:18

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Transcriptome-wide alternative splicing modulation during plant-pathogen interactions in wheat.

Hong Zhang1, Rui Mao2, Yanzhen Wang1

  • 1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, PR China.

Plant Science : an International Journal of Experimental Plant Biology
|September 16, 2019
PubMed
Summary
This summary is machine-generated.

Wheat (Triticum aestivum L.) alternative splicing (AS) diversifies transcripts and proteins for stress adaptation. This study identified AS genes responding to fungal pathogens, revealing distinct splice variants against different rusts.

Keywords:
Alternative splicingDisease defensePowdery mildewStripe rustWheat

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Area of Science:

  • Plant molecular biology
  • Genomics
  • Biochemistry

Background:

  • Alternative splicing (AS) increases transcript and protein diversity in eukaryotes, crucial for adapting to environmental stresses.
  • Understanding AS in crops like wheat (Triticum aestivum L.) is vital for improving disease resistance.

Purpose of the Study:

  • To catalog alternative splicing (AS) genes in wheat in response to fungal pathogen infections.
  • To investigate the specific AS events induced by powdery mildew (Bgt) and stripe rust (Pst) fungi.

Main Methods:

  • Characterization of 45 RNA-sequencing (RNA-seq) libraries from wheat seedlings infected with Bgt or Pst.
  • Analysis of gene expression profiles and alternative splicing events, including intron retention.

Main Results:

  • 11.2% and 10.4% of multiexon genes exhibited AS during Bgt and Pst infections, respectively.
  • Wheat modulated AS in disease resistance proteins and splicing factors, with differential expression profiles altered by splicing ratios.
  • Specific splice variants were induced against Bgt and Pst, with distinct patterns observed between resistant and susceptible plants.

Conclusions:

  • Wheat employs distinct alternative splicing strategies to combat different fungal pathogens.
  • AS plays a significant role in wheat's response to pathogen attack, influencing pathways related to defense, protein modification, translation, and transport.