Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation07:19

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation

33.0K
A tissue preparation is described for visualization and experimental manipulation of the living microcirculation. In anesthetized male mice, the thin, highly vascularized cremaster muscle is prepared for intravital microscopy to study microvascular networks including arterioles, capillaries and venules. This preparation is readily adapted for rats and...
33.0K
Psychoneuroimmunology: Cardiovascular Disease01:27

Psychoneuroimmunology: Cardiovascular Disease

414
Psychoneuroimmunology (PNI) is a multidisciplinary field that examines how psychological factors, particularly stress, interact with the immune system and impact physical health. Research in PNI has shown that chronic or traumatic stress can disrupt both the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system. These disruptions contribute to serious health conditions, including cardiovascular diseases.
A key area of focus in PNI is the relationship between stress and coronary...
414
In Silico Clinical Trials for Cardiovascular Disease09:09

In Silico Clinical Trials for Cardiovascular Disease

2.2K
This protocol demonstrates the workflow of the SILICOFCM platform for automatically generating a parametric model of the left ventricle from patient-specific ultrasound images by applying a multi-scale electromechanical model of the heart. This platform enables in silico clinical trials intended to reduce real clinical trials and maximize positive therapeutic...
2.2K
Fundus Photography as a Convenient Tool to Study Microvascular Responses to Cardiovascular Disease Risk Factors in Epidemiological Studies10:11

Fundus Photography as a Convenient Tool to Study Microvascular Responses to Cardiovascular Disease Risk Factors in Epidemiological Studies

19.6K
Retinal image analysis is an unobtrusive procedure for visualizing the microcirculation. The impact of cardiovascular disease risk factors can result in changes of retinal vessel calibers. The procedures to acquire fundus images and the steps for calculating the vessel calibers are...
19.6K
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease04:41

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

8.6K
Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular...
8.6K
Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window08:37

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

18.0K
Intravital microscopy to follow temporal and spatial hemodynamic and inflammatory events in the pial...
18.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microcirculatory response and leukocyte activation in patients undergoing elective cardiac surgery with cardiopulmonary bypass.

Journal of intensive medicine·2026
Same author

What every intensivist should know about the endothelial glycocalyx.

Journal of critical care·2026
Same author

Protective Effects of Pentoxifylline on Peripheral Microcirculatory Dysfunction and Renal Cortical Oxygen Deficiency in a Rat Model of LPS-Induced Sepsis.

Microcirculation (New York, N.Y. : 1994)·2026
Same author

Microcirculatory perfusion is improved with plasma, but not with crystalloids, in a rat polytraumatic shock model.

Transfusion·2026
Same author

Extending the frontier of tissue perfusion to monitoring the microcirculation.

Intensive care medicine·2026
Same author

Improved Detection of Capillaries in High-Resolution Handheld Vital Microscopy by Use of the MicroTools Advanced Computer Vision Algorithm.

Microcirculation (New York, N.Y. : 1994)·2026
Same journal

An Insight Into a Potential Antithrombin-Independent Mechanism of Inadequate Heparin Response in Neonates and Infants Undergoing Pediatric Cardiac Surgery: An Exploratory Study.

Journal of cardiothoracic and vascular anesthesia·2026
Same journal

The Efficacy and Safety of Weighted Blankets to Reduce Agitation After Cardiac Catheterization and Electrophysiology Procedures in Pediatric Patients With Congenital Heart Disease.

Journal of cardiothoracic and vascular anesthesia·2026
Same journal

Echocardiographic Assessment of the Mitral Annular Dimensions for Annuloplasty Ring Sizing: A Comparative Clinical Study of Two- and Three-Dimensional Ultrasound.

Journal of cardiothoracic and vascular anesthesia·2026
Same journal

Veno-Arterial Extracorporeal Membrane Oxygenation in High-Risk Ventricular Tachycardia Ablation: Current Evidence, Patient Selection, and Perioperative Management.

Journal of cardiothoracic and vascular anesthesia·2026
Same journal

Combined Observations From Two Tertiary Centers Reveal Critical Limitations in a Filtration-Based Autotransfusion System During Complex Cardiac Surgery.

Journal of cardiothoracic and vascular anesthesia·2026
Same journal

Comparison of Central and Peripheral Extracorporeal Membrane Oxygenation Cannulation Strategies in Postcardiotomy Shock.

Journal of cardiothoracic and vascular anesthesia·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
07:19

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation

Published on: June 10, 2011

33.0K

Microcirculation in Cardiovascular Diseases.

Augusto Passoni Slovinski1, Ludhmila Abrahão Hajjar1, Can Ince2

  • 1Department of Intensive Care, Cancer Institute, University of São Paulo, São Paulo, Brazil.

Journal of Cardiothoracic and Vascular Anesthesia
|September 16, 2019
PubMed
Summary
This summary is machine-generated.

Hand-held video-microscopy (HVM) enables real-time bedside analysis of microcirculation, improving hemodynamic resuscitation in critical care. This technology aids in individualized treatment, moving beyond traditional macrohemodynamic variables to optimize patient outcomes.

Keywords:
Cytocam-IDFcardiologic critical carecoronary microcirculationheart surgerymicrocirculationoxygen transportshock

More Related Videos

In Silico Clinical Trials for Cardiovascular Disease
09:09

In Silico Clinical Trials for Cardiovascular Disease

Published on: May 27, 2022

2.2K
Psychoneuroimmunology: Cardiovascular Disease
01:27

Psychoneuroimmunology: Cardiovascular Disease

414

Related Experiment Videos

Last Updated: Jan 19, 2026

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation
07:19

The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation

Published on: June 10, 2011

33.0K
In Silico Clinical Trials for Cardiovascular Disease
09:09

In Silico Clinical Trials for Cardiovascular Disease

Published on: May 27, 2022

2.2K
Psychoneuroimmunology: Cardiovascular Disease
01:27

Psychoneuroimmunology: Cardiovascular Disease

414

Area of Science:

  • Physiology
  • Critical Care Medicine
  • Medical Technology

Background:

  • Microcirculation is vital for oxygen delivery, and its disturbance is a key mortality factor in critical illnesses like sepsis and heart failure.
  • Traditional resuscitation relies on macrohemodynamic variables, risking under or over-resuscitation.
  • Early detection and management of microcirculatory dysfunction are crucial for patient survival.

Purpose of the Study:

  • To highlight the role of hand-held video-microscopy (HVM) in assessing microcirculatory variables at the bedside.
  • To demonstrate how HVM facilitates a more individualized approach to hemodynamic resuscitation in cardiac intensive care units.
  • To explore the clinical applications of HVM in managing shock, guiding therapy, and evaluating treatment response.

Main Methods:

  • Real-time analysis of microcirculatory variables using hand-held video-microscopy (HVM).
  • Bedside assessment of hemodynamic status in patients within a cardiac intensive care unit.
  • Application of HVM data to guide therapeutic interventions and treatment evaluation.

Main Results:

  • HVM allows for direct, real-time visualization and analysis of the microcirculation at the patient's bedside.
  • This technology provides critical information for classifying shock, optimizing vasopressor/inotrope dosage, and guiding fluid/transfusion therapy.
  • HVM enables objective evaluation of treatment effectiveness, promoting a demand-based and individualized resuscitation strategy.

Conclusions:

  • Hand-held video-microscopy represents a significant advancement in monitoring and managing microcirculatory dysfunction in critical care.
  • HVM supports personalized hemodynamic management, potentially improving outcomes in patients with sepsis, heart failure, and shock.
  • Further integration and validation are needed for HVM to become a standard of care in intensive care settings.