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Related Experiment Video

Updated: Jan 19, 2026

Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell
05:00

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Plasmonic droplet screen for single-cell secretion analysis.

Shih-Chung Wei1, Myat Noe Hsu2, Chia-Hung Chen3

  • 1Institute for Health Innovation & Technology, National University of Singapore, 117599, Singapore.

Biosensors & Bioelectronics
|September 16, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a novel plasmonic droplet screen for high-throughput, single-cell secretion analysis. The washing-free method enables multiplex detection of proteins like IL-8 and VEGF, advancing cellular functionality studies.

Keywords:
ImmunoassayMultiplex detectionPlatform engineeringSingle cell analysisSurface plasmon resonance

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Single-cell secretion analysis is crucial for understanding cellular heterogeneity.
  • Existing microwell assays have limited throughput, while droplet assays face challenges with washing steps.
  • High-throughput methods are needed for efficient single-cell screening.

Purpose of the Study:

  • To develop a high-throughput, washing-free method for multiplex single-cell secretion analysis.
  • To enable efficient detection of secreted proteins from individual cells.
  • To overcome limitations of current single-cell secretion analysis techniques.

Main Methods:

  • Developed a plasmonic droplet screen using antibody-conjugated gold nanorods (AuNRs).
  • Encapsulated individual cells with AuNRs within droplets for secretion evaluation.
  • Utilized dark-field spectroscopy to collect plasmonic signals from flowing single-cell droplets.

Main Results:

  • Achieved one-step, washing-free multiplex detection of single-cell secretions.
  • Demonstrated that plasmon resonance peak shift correlates with secreted protein amount.
  • Successfully screened interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) from leukemia and breast cancer cells at rates of 100-150 cells/min.

Conclusions:

  • The plasmonic droplet screen offers a novel, efficient strategy for multiplex single-cell secretion analysis.
  • This platform shows potential for high-efficiency analysis of cellular secretions.
  • The technology provides flexibility for various cell types and secreted analytes.