Cultivation technology development of Rhodothermus marinus DSM 16675

This work presents an evaluation of batch, fed-batch, and sequential batch cultivation techniques for production of R. marinus DSM 16675 and its exopolysaccharides (EPSs) and carotenoids in a bioreactor, using lysogeny broth (LB) and marine broth (MB), respectively, in both cases supplemented with 10 g/L maltose. Batch cultivation using LB supplemented with maltose (LB_malt) resulted in higher cell density (OD₆₂₀ = 6.6) than use of MB_malt (OD₆₂₀ = 1.7). Sequential batch cultivation increased the cell density threefold (OD₆₂₀ = 20) in LB_malt and eightfold (OD₆₂₀ = 14) in MB_malt. In both single and sequential batches, the production of carotenoids and EPSs using LB_malt was detected in the exponential phase and stationary phase, respectively, while in MB_malt formation of both products was detectable in both the exponential and stationary phases of the culture. Heteropolymeric EPSs were produced with an overall volumetric productivity (Q_E) of 0.67 (mg/L h) in MB_malt and the polymer contained xylose. In LB, Q_E was lower (0.1 mg/L h) and xylose could not be detected in the composition of the produced EPSs. In conclusion, this study showed the importance of a process design and medium source for production of R. marinus DSM 16675 and its metabolites.