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Related Experiment Video

Updated: Jan 19, 2026

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
12:36

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

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Immunophenotyping by Mass Cytometry.

Gregory K Behbehani1

  • 1Department of Internal Medicine, Division of Hematology, James Cancer Hospital, The Ohio State University, Columbus, OH, USA. Gregory.behbehani@osumc.edu.

Methods in Molecular Biology (Clifton, N.J.)
|September 16, 2019
PubMed
Summary
This summary is machine-generated.

Mass cytometry uses metal-tagged antibodies and mass spectrometry for high-dimensional analysis. This novel technology allows for detailed immunophenotyping and functional profiling of complex biological samples.

Keywords:
Aberrant marker expressionAcute leukemiaCyTOFFlow cytometryImmunophenotypingMass cytometryMinimal residual disease

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Area of Science:

  • Biotechnology
  • Immunology
  • Analytical Chemistry

Background:

  • Flow cytometry is a standard technique for cell analysis.
  • Traditional methods face limitations in multiplexing and background noise.
  • There is a need for advanced technologies to analyze complex samples.

Purpose of the Study:

  • To introduce mass cytometry as a powerful tool for biological research.
  • To describe methods for immunophenotyping using mass cytometry.
  • To explain the integration of functional assays with mass cytometry.

Main Methods:

  • Antibodies are conjugated to heavy metal isotopes.
  • Detection is performed using time-of-flight mass spectrometry.
  • Simultaneous measurement of up to 50 parameters per cell.

Main Results:

  • Mass cytometry offers high-dimensional data acquisition (40-50 markers).
  • It eliminates autofluorescent background and minimizes signal spillover.
  • Enables precise immunophenotyping of complex samples.

Conclusions:

  • Mass cytometry significantly advances cell analysis capabilities.
  • It provides a robust platform for complex sample interrogation.
  • Facilitates combined immunophenotyping and functional cellular analysis.