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Updated: Jan 19, 2026

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
Published on: November 4, 2016
Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric Analysis
Abdullah Alsuwaidan1, Franklin Fuda2, Weina Chen2
1Division of Molecular and Genomic Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.
Abstract:
Dendritic cells (DCs) are powerful antigen presenting cells that are involved in regulating immune response. Plasmacytoid dendritic cells (pDCs) are subtype of DCs that present in small quantity in the bone marrow, peripheral blood, and lymph nodes. They are important component of our immune system in normal condition and diseases. They activate T cells and play a critical role in immune tolerance. In this chapter we review the immunophenotypic features of pDCs and provide a practical protocol for pDCs enumeration in the peripheral blood and bone marrow samples.
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