Influence of miR-376c-3p/SYF2 Axis on the Progression of Gastric Cancer

Area of Science:

• Molecular Biology
• Oncology
• Gene Regulation

Background:

• MicroRNA-376c-3p is implicated in human cancer progression.
• Gastric cancer remains a significant global health concern, necessitating research into its underlying mechanisms.

Purpose of the Study:

• To investigate the role of microRNA-376c-3p in human gastric cancer cell proliferation and migration.
• To elucidate the molecular mechanism by which microRNA-376c-3p influences gastric cancer progression, focusing on its interaction with SYF2.

Main Methods:

• Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to assess microRNA-376c-3p expression levels in gastric cancer cell lines versus normal cells.
• The direct targeting relationship and functional impact of microRNA-376c-3p on SYF2 (pre-mRNA splicing factor) were analyzed.
• Gain-of-function experiments involving microRNA-376c-3p mimic and SYF2 overexpression were performed to evaluate effects on cell behaviors.

Main Results:

• MicroRNA-376c-3p expression was significantly downregulated in human gastric cancer cell lines compared to normal gastric cells.
• MicroRNA-376c-3p was identified as a direct targeting molecule of SYF2, leading to reduced SYF2 expression.
• Overexpression of microRNA-376c-3p suppressed gastric cancer cell proliferation and migration.
• Conversely, overexpression of SYF2 counteracted the inhibitory effects of microRNA-376c-3p on gastric cancer cell behaviors.

Conclusions:

• MicroRNA-376c-3p functions as a tumor suppressor in gastric cancer.
• The inhibitory effect of microRNA-376c-3p on gastric cancer cell proliferation and migration is mediated through the direct targeting and downregulation of SYF2.