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Related Experiment Video

Updated: Jan 19, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Structural mass spectrometry goes viral.

Jasmin Dülfer1, Alan Kadek2, Janine-Denise Kopicki1

  • 1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.

Advances in Virus Research
|September 17, 2019
PubMed
Summary

Mass spectrometry (MS) is revolutionizing structural virology by analyzing viral protein structure and dynamics. Advanced MS techniques now enable studying intact viruses and virus-host interactions at the cellular level.

Keywords:
Capsid assemblyCharge detection mass spectrometry (CDMS)Fast photochemical oxidation of proteins (FPOP)Gas-phase electrophoretic mobility analysis (GEMMA)Ion mobility (IM)Protein conformational dynamicsStructural virology

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Area of Science:

  • Structural biology
  • Virology
  • Biophysics

Background:

  • Mass spectrometry (MS) offers high speed and sensitivity for analyzing small sample amounts.
  • MS is increasingly utilized in structural virology to study viral proteins in native-like environments.

Purpose of the Study:

  • To review recent advances in mass spectrometry approaches for structural virology.
  • To highlight the integration of MS with other biophysical techniques for modeling viral assemblies.
  • To showcase the shift towards systems biology and cellular-level experiments in structural virology.

Main Methods:

  • Hydrogen-deuterium exchange MS for analyzing protein interactions and dynamics.
  • Cross-linking MS for studying viral protein complexes and virus-host interactions.
  • Native MS for gas-phase analysis of viral proteins, complexes, and intact virions.

Main Results:

  • MS techniques provide insights into the size, stability, interactions, and dynamics of virus assemblies.
  • MS data offer constraints for integrative structural modeling of complex viral structures.
  • Advanced MS enables the analysis of intact virions and virus-host interactions within cells.

Conclusions:

  • Structural MS approaches are advancing structural virology towards a systems biology perspective.
  • MS is becoming a powerful tool for studying viral structures and dynamics at the cellular level.
  • The integration of MS with other techniques enhances the understanding of viral complex assemblies.