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Related Concept Videos

Microtubules01:35

Microtubules

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There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.
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Microtubules01:18

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Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
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Microtubules, which are tubulin polymers, play a crucial role as a cytoskeleton component in eukaryotic cells and are known for their dynamic instability. This study developed a method for fractionating microtubules to separate them into stable microtubules, labile microtubules, and free tubulin to evaluate the stability of microtubules in various mouse...
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Microtubules are inherently unstable polymers, and their switching between growth and shortening is stochastic and difficult to control. Here we describe protocols using segmented microtubules with photoablatable stabilizing caps. Depolymerization of segmented microtubules can be triggered with high temporal and spatial resolution, thereby assisting analysis of motions with the disassembling microtubule...
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Self-Assembly of Microtubule Tactoids08:49

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This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding...
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Here, we describe a protocol to extract endogenous tubulin from mammalian cells, which can lack or contain specific microtubule-modifying enzymes, to obtain microtubules enriched for a specific modification. We then describe how the extracted microtubules can be decorated with purified microtubule-binding proteins to prepare grids for cryo-electron...
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Related Experiment Video

Updated: Jan 19, 2026

Microtubules and Cell Motility
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Microtubules and Cell Motility

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A Microtubule-Myelination Connection.

Antonina Roll-Mecak1

  • 1Cell Biology and Biophysics Unit, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, USA; Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, USA.

Cell
|September 17, 2019
PubMed
Summary
This summary is machine-generated.

Researchers identified a key regulator of microtubule growth in oligodendrocytes. This protein promotes microtubule extension from Golgi outposts, controlling myelin sheath elongation and central nervous system myelination.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Microtubules are essential for oligodendrocyte process extension and myelin deposition.
  • The precise mechanisms of microtubule biogenesis in oligodendrocytes remain largely unknown.

Purpose of the Study:

  • To identify novel regulators of microtubule dynamics in oligodendrocytes.
  • To elucidate the role of these regulators in myelin sheath formation and CNS myelination.

Main Methods:

  • Oligodendrocyte culture and manipulation.
  • Microtubule dynamics imaging.
  • Analysis of myelin sheath formation in vivo and in vitro.

Main Results:

  • Identification of an oligodendrocyte-enriched microtubule regulator.
  • Demonstration that this regulator promotes microtubule growth from Golgi outposts.
  • Evidence linking the regulator to control over myelin sheath elongation.

Conclusions:

  • The identified regulator is crucial for microtubule organization in oligodendrocytes.
  • This protein plays a significant role in linking microtubule cytoarchitecture to CNS myelination.