OLICH: A reference library of DNA barcodes for Nordic lichens

Area of Science:

• Mycology
• Genomics
• Biodiversity research

Background:

• DNA barcoding is a crucial tool for species identification in lichenized fungi.
• A comprehensive DNA barcode sequence library is needed for Nordic lichens.

Purpose of the Study:

• To create an authoritative DNA barcode sequence library for Nordic lichens.
• To facilitate accurate species identification and taxonomic research in the region.

Main Methods:

• Sequencing of the nrITS region for DNA barcoding.
• Data compilation and analysis for a wide range of Nordic lichen species.

Main Results:

• 1324 DNA barcode sequences (nrITS) for 507 species across 175 genera and 25 orders were generated.
• 38 species are newly added to GenBank, with ITS sequences for 25 additional species presented for the first time.
• The dataset represents 20-21% of known Nordic lichenized species.

Conclusions:

• The generated dataset significantly expands the available DNA barcode data for Nordic lichens.
• Barcode gap analyses were performed for genera Cladonia, Ramalina, and Umbilicaria.
• Taxonomic revisions were proposed, including a new combination and reclassifications of several species.