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Related Concept Videos

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Related Experiment Video

Updated: Jan 19, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
08:51

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Published on: November 4, 2016

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Spa Typing of Staphylococcus aureus Isolates.

Xin Wang1

  • 1College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China. xinwang7516@nwusaf.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|September 17, 2019
PubMed
Summary

Staphylococcal protein A (SPA) typing is a genotyping method that analyzes gene variations in the X-region. This rapid technique predicts other typing methods and facilitates easy data sharing online.

Keywords:
PCR amplification of spa geneSpa typingStaphylococcus aureusspa Gene sequencing

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Area of Science:

  • Microbiology
  • Genetics
  • Bioinformatics

Background:

  • Staphylococcal protein A (SPA) is a surface protein with distinct regions: Fc-partial-region, X-region, and C-terminal.
  • The X-region contains variable 24 bp repeat sequences, forming the basis for SPA typing.

Purpose of the Study:

  • To describe SPA typing as a genotyping method.
  • To highlight its utility in characterizing and predicting other microbial typing methods.
  • To emphasize the advantages of DNA sequence-based approaches in microbiology.

Main Methods:

  • Spa typing based on gene polymorphism in the variable number of 24 bp repeat sequences within the X-region of Staphylococcal protein A.
  • Utilizing DNA sequence data for characterization and inter-laboratory data transfer.

Main Results:

  • Spa typing provides highly discriminatory and rapid characterization of bacterial strains.
  • It enables accurate prediction of other established typing methods like MLST, ribotyping, and PFGE.
  • DNA sequence-based methods offer ease of data transfer via the internet.

Conclusions:

  • Spa typing is an effective and efficient genotyping tool for Staphylococcal species.
  • Its ability to predict other typing methods streamlines microbial analysis.
  • The adoption of DNA sequence-based approaches, facilitated by internet transfer, enhances global research collaboration.