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Microfluidic quantification and separation of yeast based on surface adhesion.

Kristina Reinmets1, Amin Dehkharghani, Jeffrey S Guasto

  • 1Department of Biology, Tufts University, Medford, MA 02155, USA. Stephen.fuchs@tufts.edu.

Lab on a Chip
|September 17, 2019
PubMed
Summary

We developed a microfluidic assay to quantify yeast adhesion, enabling strain separation and property screening for diverse applications like bioengineering and infection control.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Surface Science

Background:

  • Fungal adhesion is critical in various fields, including infections, food production, and bioengineering.
  • Current methods for quantifying yeast surface adhesion at a population scale are insufficient.

Purpose of the Study:

  • To develop a robust method for quantifying yeast surface adhesion.
  • To enable discrimination and separation of yeast strains based on adhesion strength.
  • To investigate the impact of ionic strength and substrate hydrophobicity on yeast adhesion.

Main Methods:

  • A novel microfluidic assay was designed and implemented.
  • The assay differentiates and separates genetically related yeast strains by adhesion strength.
  • Experiments were conducted to assess the effects of varying ionic strength and substrate hydrophobicity.

Main Results:

  • The microfluidic assay successfully quantified yeast adhesion strength.
  • The assay allowed for the discrimination and separation of yeast strains based on adhesion.
  • Ionic strength and substrate hydrophobicity were shown to significantly influence yeast adhesion.

Conclusions:

  • The developed microfluidic assay provides a powerful tool for population-scale quantification of yeast adhesion.
  • This method facilitates rapid screening and fractionation of yeast based on adhesive properties.
  • Applications include genetic protein engineering, development of anti-fouling surfaces, and understanding fungal pathogenesis.