Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

32.4K
This islet isolation protocol described a novel route of collagenase injection to digest the exocrine tissue and a simplified gradient procedure to purify the islets from mice. It involves enzymatic digestion, gradient separation/purification, and islet hand-picking. Successful isolation can yield 250–350 high quality and fully functional islets per...
32.4K
A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice14:39

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice

218
This study developed a simplified method to efficiently extract functional primary islets and acinar cells from the mouse pancreas, offering a valuable tool for studying intercellular communication in the pathogenesis of Acute Pancreatitis-Induced...
218
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells07:21

Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells

4.8K
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from...
4.8K
Isolated Pancreatic Islet Treatment and Apoptosis Measurement09:36

Isolated Pancreatic Islet Treatment and Apoptosis Measurement

858
This study utilizes multi-staining fluorescence-based markers of cell death and apoptosis combined with confocal microscopy to assess cytokine-induced apoptosis and β-cell-specific death in pancreatic islets. It reveals spatial and temporal changes in cell death and apoptosis in response to extracellular...
858
Intraportal Transplantation of Pancreatic Islets in Mouse Model09:01

Intraportal Transplantation of Pancreatic Islets in Mouse Model

10.2K
Pancreatic islet transplantation is a way to achieve normoglycemia in type 1 diabetes. This article focuses on the transplantation technique through the intraportal route to maintain normal glucose levels in diabetic...
10.2K
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals07:39

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

7.7K
Here we present a protocol to isolate mouse pancreatic islet cells for screening the ROS inductions by the xenobiotics in order to identify the potential diabetogenic xenobiotic...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of High-Frequency Low-Tidal Volume Versus Jet Ventilation on Acute and Long-Term Outcomes Following Pulmonary Vein Isolation.

Journal of cardiovascular electrophysiology·2026
Same author

A community-based intervention using a board game to improve traffic safety behaviors among older adults: a quasi-experimental study.

BMC geriatrics·2026
Same author

Brain health and the gut microbiome (bMicrobiome Study): a proof-of-concept, feasibility study integrating shotgun metagenomics, metrology, and multidimensional phenotyping across the cognitive aging spectrum.

Gut microbes reports·2026
Same author

Spider venom peptides Ht1a and Gg1a are toxic to honeybee parasite <i>Varroa destructor</i> by topical application.

npj drug discovery·2026
Same author

Pulsed-Field Ablation for Persistent Atrial Fibrillation: A Systematic Review and Meta-Analysis.

Journal of cardiovascular electrophysiology·2026
Same author

Hepatic TGFβ1 signaling impairs insulin sensitivity via inducing insulin receptor substrate 1 degradation.

Metabolism: clinical and experimental·2026

Related Experiment Video

Updated: Jan 19, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

32.4K

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice.

Daniel Villarreal1, Geetali Pradhan2, Chia-Shan Wu3

  • 1Department of Nutrition and Food Science, Texas A&M University.

Journal of Visualized Experiments : Jove
|September 17, 2019
PubMed
Summary

This study presents a simplified, high-yield method for isolating pancreatic islets, crucial for diabetes research. The improved protocol ensures better quality islets for ex vivo studies by optimizing digestion and purification steps.

More Related Videos

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice
14:39

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice

Published on: January 9, 2026

218
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
07:21

Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells

Published on: April 30, 2023

4.8K

Related Experiment Videos

Last Updated: Jan 19, 2026

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice
06:56

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Published on: August 30, 2019

32.4K
A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice
14:39

A Simple and Rapid Method for Simultaneous Isolation of Primary Islets and Primary Pancreatic Acinar Cells from Mice

Published on: January 9, 2026

218
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
07:21

Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells

Published on: April 30, 2023

4.8K

Area of Science:

  • Endocrinology
  • Cell Biology
  • Diabetes Research

Background:

  • Pancreatic islets (Islets of Langerhans) are vital endocrine cell clusters regulating glucose via hormones like insulin and glucagon.
  • High-quality islet isolation is critical for diabetes research, but current methods are complex and invasive.
  • Beta cells, the most abundant islet cells, are key to studying insulin secretion.

Purpose of the Study:

  • To introduce a simple, high-yield mouse islet isolation protocol.
  • To improve upon existing methods by simplifying injection, digestion, and purification.
  • To provide islets suitable for various ex vivo studies.

Main Methods:

  • Collagenase P injection into the accessible ampulla of Vater.
  • Combined enzymatic digestion and mechanical separation of the exocrine pancreas.
  • Single-step density gradient purification.

Main Results:

  • The protocol yields approximately 250-350 islets per mouse.
  • Islets obtained are suitable for diverse ex vivo research applications.
  • The method simplifies enzyme injection and digestion while reducing purification steps.

Conclusions:

  • This optimized protocol offers a more accessible and efficient approach to mouse islet isolation.
  • The simplified technique yields high-quality islets, beneficial for diabetes research.
  • Careful optimization of incubation time can mitigate potential islet damage.