Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface10:38

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

38.1K
Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory...
38.1K
Efficiency of Liquid-liquid Extraction09:21

Efficiency of Liquid-liquid Extraction

49.9K
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA
Liquid-liquid extraction (LLE) is a separation technique used instead of distillation when either: (a) the relative volatilities of the compounds to be separated are very similar; (b) one or more of the mixture components are temperature sensitive even near ambient conditions; (c) the distillation would require a very low pressure or a very high distillate/feed...
49.9K
Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

10.2K
We present a facile method to fabricate a biodegradable gelatin-based drug release platform that is magneto-thermally responsive. This was achieved by incorporating superparamagnetic iron oxide nanoparticles and poly(N-isopropylacrylamide-co-acrylamide) within a spherical gelatin micro-network crosslinked by genipin, in conjunction with an alternating magnetic field application...
10.2K
A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

4.6K
The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research...
4.6K
Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures13:38

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

10.0K
This study presents a methodology to prepare 3D, biodegradable, foam-like cell scaffolds based on biocompatible side-chain liquid crystal elastomers (LCEs). Confocal microscopy experiments show that foam-like LCEs allow for cell attachment, proliferation, and the spontaneous alignment of C2C12s...
10.0K
Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy11:03

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy

4.0K
Cryogenic Focused Ion Beam (FIB) and Scanning Electron Microscopy (SEM) techniques can provide key insights into the chemistry and morphology of intact solid-liquid interfaces. Methods for preparing high quality Energy Dispersive X-ray (EDX) spectroscopic maps of such interfaces are detailed, with a focus on energy storage...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amphiphilic Hollow Microgels at the Liquid-Liquid Interface: Studying Soft Colloids' Shape and Interior via Computer Simulations and Machine Learning.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Scaling Law for Sequence-Induced Demixing of Compositionally Identical Copolymers.

ACS macro letters·2026
Same author

Solutions of Carboxymethylcellulose with Organic Counterions (I): The Influence of Counterion Properties on the Polymer Structure and Solubility.

Macromolecules·2026
Same author

Correction: Harnessing the polymer-particle duality of ultra-soft nanogels to stabilise smart emulsions.

Physical chemistry chemical physics : PCCP·2025
Same author

Size and softness synergy in cellular microgel uptake: a force spectroscopy study.

Nanoscale·2025
Same author

From single microgels to dense microgel monolayers - investigation by atomic force microscopy.

Soft matter·2025

Related Experiment Video

Updated: Jan 19, 2026

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
10:38

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

Published on: October 8, 2013

38.1K

Polyelectrolyte Microgels at a Liquid-Liquid Interface: Swelling and Long-Range Ordering.

Alexey A Gavrilov1, Walter Richtering2, Igor I Potemkin1,3,4

  • 1Physics Department , Lomonosov Moscow State University , Moscow 119991 , Russian Federation.

The Journal of Physical Chemistry. B
|September 17, 2019
PubMed
Summary
This summary is machine-generated.

Charged microgels exhibit controlled interfacial ordering and swelling behavior influenced by solvent quality and charge density. Dissipative particle dynamics simulations reveal tunable microgel properties for advanced material design.

More Related Videos

Liquid-liquid Extraction and York-Scheibel Column Operation
09:21

Liquid-liquid Extraction and York-Scheibel Column Operation

Published on: April 30, 2023

49.9K
Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Published on: February 13, 2016

10.2K

Related Experiment Videos

Last Updated: Jan 19, 2026

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
10:38

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

Published on: October 8, 2013

38.1K
Liquid-liquid Extraction and York-Scheibel Column Operation
09:21

Liquid-liquid Extraction and York-Scheibel Column Operation

Published on: April 30, 2023

49.9K
Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
09:11

Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release

Published on: February 13, 2016

10.2K

Area of Science:

  • Soft Matter Physics
  • Computational Chemistry
  • Materials Science

Background:

  • Charged microgels are versatile materials with applications in drug delivery, sensors, and coatings.
  • Understanding microgel behavior at interfaces is crucial for designing advanced functional materials.
  • Liquid-liquid interfaces present unique environments that influence microgel structure and dynamics.

Purpose of the Study:

  • To investigate the behavior of charged microgels at liquid-liquid interfaces using simulations.
  • To determine the effects of charge fraction and solvent quality on microgel properties.
  • To explore the 2D ordering of microgels at the interface.

Main Methods:

  • Dissipative Particle Dynamics (DPD) simulations with explicit electrostatic interactions.
  • Modeling of polar and nonpolar solvents (e.g., water and oil).
  • Analysis of microgel size, shape, interfacial adsorption, and ordering.

Main Results:

  • Increasing charge fraction leads to microgel swelling in polar solvents and reduced adsorption.
  • Microgels detach into polar solvents when the nonpolar solvent quality is poor.
  • Microgel swelling is dependent on solvent quality and temperature (for thermosensitive microgels).
  • Implicit and explicit dielectric permittivity models show qualitative agreement, with explicit models necessary for interfacial phenomena.
  • Charged microgels form ordered hexagonal packing at interfaces, unlike random uncharged microgels.

Conclusions:

  • Microgel interfacial behavior is highly tunable via charge density and solvent interactions.
  • Ordered microgel assemblies at interfaces can be achieved even at low charge fractions.
  • The findings suggest potential for pH-sensitive microgels in creating controlled interfacial structures.