Enhanced Recovery After Head and Neck Cancer Reconstruction With a Free Flap-What Is Next?

Here we present a protocol for the use of the pig superior epigastric artery perforator flap as a learning module for head and neck microvascular...

This protocol presents a novel coronoid-temporalis pedicled flap (CTPF) technique for reconstructing small-to-medium orbital floor defects. CTPF avoids microvascular risks while maintaining biomechanical stability. Clinical cases demonstrate effective structural restoration, reduced complications, and preserved facial symmetry, offering a viable alternative to traditional grafts or alloplastic materials.

This protocol presents a step-by-step instruction for harvesting the superficial inferior epigastric artery fascia (SIEF) flap and wrapping it around the reconstructed peroneal nerve defect in rabbits. This surgical addition of vascularization to the tissue bed of nerve grafts is used to improve regeneration outcomes.

The human body's intricate network of arteries ensures that every organ system receives the necessary oxygen and nutrients for optimal function. The arterial network in the head and neck region is particularly complex, providing vital blood flow to the brain, eyes, and other critical structures. Prominent arteries in this region include the internal carotid arteries and the vertebral arteries.
The internal carotid arteries supply blood to the anterior portion of the cerebrum. They enter the...

The blood drainage from the head and neck is primarily managed by three pairs of veins: the external jugular, internal jugular, and vertebral veins. The external jugular veins drain superficial scalp and face structures, passing over the sternocleidomastoid muscles to empty into the subclavian veins.
On the other hand, the vertebral veins, unlike their arterial counterparts, are not primarily responsible for brain drainage. Instead, they drain the cervical vertebrae, spinal cord, and some small...

Development of murine models with specific genes mutated in head and neck cancer patients is required for understanding of neoplasia. Here, we present a protocol for in vitro transformation of primary murine tongue cells using an adeno-associated virus-Cas9 system to generate murine HNC cell lines with specific genomic...