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Updated: Jan 19, 2026

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
Published on: September 13, 2021
Electron cryo-microscopy for elucidating the dynamic nature of live-protein complexes
1RIKEN SPring-8 Center, Hyogo, Japan.
Background:
Recent innovations in the field of electron cryo-microscopy (cryo-EM) have achieved structure determination of target macromolecules at "near-atomic resolution". In addition, cryo-EM has the potential to deal with proteins in multiple conformations in close to physiological conditions.
Scope Of Review:
This is an overview of the latest technical and methodological developments in cryo-EM, especially key features for elucidating the dynamic nature of specimens.
Major Conclusions:
It is now possible to elucidate "near-atomic resolution" structures by selecting "well-behaved" particles in Single Particle Analysis (SPA) of cryo-EM images.
General Significance:
Currently, cryo-EM is utilized actively as an alternative to X-ray crystallography to obtain high-resolution structures of macromolecules, especially those which are hard to crystalize. One possible reason for difficulty in crystallization could be the dynamic nature of the specimen. New developments in the field of cryo-EM will make it possible to deal with specimen heterogeneity and reveal the dynamic nature of specimens.
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