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A Simplified Method for Isolation and Culture of Retinal Pigment Epithelial Cells from Adult Mice

Retinal pigment epithelium (RPE) acts as a crucial barrier between the choroid and retina, promoting the health and function of retinal cell types, such as photoreceptors. Herein, we describe a simple and effective protocol for isolating and culturing adult murine...
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Reaction Stoichiometry02:57

Reaction Stoichiometry

A balanced chemical equation provides a great deal of information in a very succinct format. Chemical formulas provide the identities of the reactants and products involved in the chemical change, allowing classification of the reaction. Coefficients provide the relative numbers of these chemical species, allowing a quantitative assessment of the relationships between the amounts of substances consumed and produced by the reaction. These quantitative relationships are known as the reaction’s...
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A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening08:34

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

A protocol for small molecular drug screening based on in-situ synthesis of ultrasmall fluorescent gold nanoclusters (Au NCs) using drug-loaded protein as template is presented. This method is simple to determine the binding affinity of drugs to a target protein by a visible fluorescent signal emitted from the protein-templated Au...
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A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry09:54

A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry

The goal of this procedure is to demonstrate the reproducibility and adaptability of using a microtiter plate format for microalgal screening. This rapid screen combines WATER-Pulse-Amplitude-Modulated (WATER-PAM) fluorometry to measure photosynthetic yield as an indicator of Photosystem II (PSII) health with small volume bacterial-algal...
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Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy13:44

Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy

Recently, a vast amount of prospects have come available for human-robot interactive systems. In this paper we outline the integration of a new robotic device with open source software that can rapidly make possible a library of interactive functionality. We then outline a clinical application for a neurorehabilitation...
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An Introduction to the Micropipettor09:15

An Introduction to the Micropipettor

The micropipettor is a common laboratory instrument used for transferring microvolumes of liquid solutions. Micropipettors come in a range of sizes for the accurate movement of volumes between 0.5 and 5000 μl and each instrument requires one of three different sized disposable tips. Micropipettors work by displacing air from the pipette shaft, allowing the liquid to be drawn into the resulting vacuum. Their uses include transferring cell suspensions for a variety of cell-based assays,...
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