Related Concept Videos

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries07:35

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries

Here, we present a protocol demonstrating the installation and use of a bioinformatics pipeline to analyze chimeric RNA sequencing data used in the study of in vivo RNA:RNA...
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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding...
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Stereotactic Injection of Hydrogel into a Post-Stroke Mouse Model02:59

Stereotactic Injection of Hydrogel into a Post-Stroke Mouse Model

Source: Wilson, K.L., et al. Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke. J. Vis. Exp. (2020)This video demonstrates the stereotaxic injection of a hydrogel into the stroke core of a post-stroke mouse, highlighting its role in scaffold formation that mimics the extracellular matrix, promotes cellular infiltration, and supports angiogenesis, underscoring its neurotherapeutic potential in stroke...
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Spinal Hernia Repair and Cauda Equina Repositioning After Lumbar Decompression under Three-Dimensional Microscopy: A Case Report and Literature Review04:33

Spinal Hernia Repair and Cauda Equina Repositioning After Lumbar Decompression under Three-Dimensional Microscopy: A Case Report and Literature Review

Dural hernia following spinal endoscopic surgery is a rare complication. Here, we report a case of nerve root hernia associated with the absence of dural repair, prolonged operation time, and increased abdominal pressure due to postoperative constipation. Early dural tear repair and monitoring postoperative constipation can help prevent cauda equina...
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Gathering Self-Initiated Rat Behavioral Data to Characterize Post-Stroke Deficits05:08

Gathering Self-Initiated Rat Behavioral Data to Characterize Post-Stroke Deficits

A system for acquiring data from self-initiated individual behavior sessions within a social colony cage setting is presented. The efficacy of this system is demonstrated using an automated skilled reach assessment, enabling the characterization of post-stroke motor impairments, potential behavioral alterations related to motivation, circadian variations, and other innovative dependent...
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Murine Flexor Tendon Injury and Repair Surgery07:32

Murine Flexor Tendon Injury and Repair Surgery

Flexor tendons in the hand are commonly injured, leading to impaired hand function. However, the scar-tissue healing response is not well characterized. A murine model of flexor tendon healing is demonstrated here. This model can enhance overall understanding of the healing process and assess therapeutic approaches to improve...
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