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An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart10:28

An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart

Supramolecular hydrogelators based on ureido-pyrimidinones allow full control over the macroscopic gel properties and the sol–gel switching behavior using pH. Here, we present a protocol for formulating and injecting such a supramolecular hydrogelator via a catheter delivery system for local delivery directly in relevant areas in the pig...
17.9K
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes09:21

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation...
24.7K
Mouse Round Spermatid Injection08:41

Mouse Round Spermatid Injection

Here, we introduce a method for performing round spermatid injection (ROSI) in mice, a technique with promising clinical applications and utility for investigating the mechanisms underlying embryonic...
1.4K
Facilitating Repeat Intracarotid Injections in Mouse Models by a Novel Injection Site Repair Technique09:08

Facilitating Repeat Intracarotid Injections in Mouse Models by a Novel Injection Site Repair Technique

Repair of the intracarotid artery in a mouse model after injection returns blood flow to the artery without negatively impacting the distribution of the injected material. Injection site repair facilitates subsequent injections through the same artery and prevents cerebral ischemia in mouse strains that lack a complete Circle of...
2.8K
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland03:45

Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland

This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary...
5.0K
Gas Chromatography: Sample Injection Systems01:08

Gas Chromatography: Sample Injection Systems

In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
Two primary injection methods are used...
1.4K