The Combinations Chitosan-Pam₃CSK₄ and Chitosan-Monophosphoryl Lipid A: Promising Immune-Enhancing Adjuvants for...

We previously demonstrated that recombinant protein PAc could be administered as an anticaries vaccine. However, the relatively weak immunogenicity of PAc limits its application. In the present study, we investigated the effect of two adjuvant combinations of chitosan plus Pam₃CSK₄ (chitosan-Pam₃CSK₄) and of chitosan plus monophosphoryl lipid A (chitosan-MPL) in the immune responses to the PAc protein in vivo and in vitro PAc-chitosan-Pam₃CSK₄ or PAc-chitosan-MPL promoted significantly higher PAc-specific antibody titers in serum and saliva, inhibited Streptococcus mutans colonization onto the tooth surfaces, and endowed better protection effect with significantly less caries activities than PAc alone. Chitosan-Pam₃CSK₄ and chitosan-MPL showed no statistically significant differences. In conclusion, our study demonstrated that the chitosan-Pam₃CSK₄ and chitosan-MPL combinations are promising for anticaries vaccine development.