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Recent progress in induced pluripotent stem cell-derived cardiac cell sheets for tissue engineering.

Botao Gao1, Katsuhisa Matsuura1, Tatsuya Shimizu1

  • 1Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University.

Bioscience Trends
|September 19, 2019
PubMed
Summary

Tissue engineering advances combine cell sheet technology with human induced pluripotent stem cells (hiPSC) to create functional cardiac tissues. This approach addresses challenges in large-scale cell culture and safe clinical applications for treating heart diseases.

Keywords:
Cell sheet engineeringInduced pluripotent stem cell-derived cardiac cellsLarge-scale suspension culture systems

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Area of Science:

  • Regenerative Medicine
  • Biotechnology
  • Stem Cell Biology

Background:

  • Significant advancements in tissue engineering and stem cell research have occurred over the past decade.
  • Cell sheet technology allows harvesting of confluent cells as intact sheets from temperature-responsive culture dishes.
  • Human induced pluripotent stem cells (hiPSC) offer potential for generating specific cell types for tissue regeneration and disease modeling.

Purpose of the Study:

  • To review progress in combining cell sheet technology with hiPSC-derived cardiac cells for fabricating functional human cardiac tissues.
  • To summarize challenges and solutions in large-scale hiPSC-derived cardiac cell culture.
  • To discuss methods for eliminating undifferentiated hiPSCs and myocardial tissue fabrication.

Main Methods:

  • Utilizing temperature-responsive culture dishes for cell sheet harvesting.
  • Employing human induced pluripotent stem cells (hiPSC) for cardiac cell differentiation.
  • Developing techniques for large-scale culture and purification of hiPSC-derived cardiac cells.
  • Fabricating myocardial tissues using cell sheet technology.

Main Results:

  • Successful creation of multiple cell sheet-based tissues for various disease therapies, particularly myocardial diseases.
  • Demonstrated combination of cell sheet technology and hiPSC-derived cardiac cells for functional cardiac tissue fabrication.
  • Progress in addressing challenges related to large-scale cell culture and differentiation purity.

Conclusions:

  • The integration of cell sheet technology and hiPSC-derived cardiac cells shows promise for regenerating functional cardiac tissues.
  • Addressing challenges in cell culture scale-up, purity, and fabrication is crucial for clinical translation.
  • This approach holds potential for treating myocardial diseases, in vitro drug screening, and disease modeling.