Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibody-Free Assay for RNA Methyltransferase Activity Analysis08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

7.6K
Here, an antibody-free in vitro assay for direct analysis of methyltransferase activity on synthetic or in vitro transcribed RNA is...
7.6K
In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity09:33

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

10.2K
The present report describes an in vitro enzymatic assay to measure phosphatidylethanolamine methyltransferase activity using Leishmania cell extracts. This assay is based on the transfer of a radioactive methyl group from S-[Methyl-3H]adenosyl-L-methionine onto endogenous phosphatidylethanolamine.
10.2K
Requirements for Human Life01:26

Requirements for Human Life

13.0K
The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
13.0K
Asthma: Pathogenesis and Management01:20

Asthma: Pathogenesis and Management

1.2K
Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
1.2K
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

735
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
735
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR12:32

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

13.2K
Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine...
13.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction Note: NSD2 targeting reverses plasticity and drug resistance in prostate cancer.

Nature·2026
Same author

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same author

Dissecting the host determinants of orthoflavivirus infection using QIC-seq.

PLoS pathogens·2026
Same author

Secreted ORF8 reprograms macrophages to enhance SARS-CoV-2 infection of lung epithelial cells.

bioRxiv : the preprint server for biology·2026
Same author

A proteogenomic RNA processing mechanism drives sex differences in meningioma.

Research square·2026
Same author

Human vaccine responses regulated by parallel cytokine pathways.

Nature immunology·2026

Related Experiment Video

Updated: Jan 19, 2026

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.6K

Enterovirus pathogenesis requires the host methyltransferase SETD3.

Jonathan Diep1, Yaw Shin Ooi1, Alex W Wilkinson2

  • 1Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA.

Nature Microbiology
|September 19, 2019
PubMed
Summary

The actin histidine methyltransferase SET domain containing 3 (SETD3) is essential for enterovirus (EV) replication and pathogenesis. SETD3 interacts with the viral 2A protease, facilitating viral RNA replication and suggesting SETD3 as a therapeutic target.

More Related Videos

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
09:33

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

Published on: January 5, 2016

10.2K
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
12:32

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Published on: January 16, 2016

13.2K

Related Experiment Videos

Last Updated: Jan 19, 2026

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.6K
In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
09:33

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

Published on: January 5, 2016

10.2K
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
12:32

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Published on: January 16, 2016

13.2K

Area of Science:

  • Virology
  • Molecular Biology
  • Host-Pathogen Interactions

Background:

  • Enteroviruses (EVs) cause significant human diseases, but mechanisms of viral replication are not fully understood.
  • Identifying host factors essential for EV replication is crucial for developing antiviral strategies.

Purpose of the Study:

  • To identify host proteins critical for enterovirus replication using genome-scale CRISPR screens.
  • To elucidate the role of SETD3 in the enterovirus life cycle and its interaction with viral proteins.

Main Methods:

  • Genome-scale CRISPR screens to identify host factors.
  • Quantitative affinity purification-mass spectrometry to identify protein interactions.
  • Site-directed mutagenesis to study protein function.
  • In vivo mouse models to assess viral pathogenesis.

Main Results:

  • SET domain containing 3 (SETD3) was identified as essential for replication of a broad range of EVs.
  • SETD3, independent of its methylation activity, is required for viral RNA replication.
  • SETD3 specifically interacts with the viral 2A protease, and this interaction is critical for RNA replication.
  • SETD3 is essential for EV replication and pathogenesis in vivo.

Conclusions:

  • SETD3 plays a critical role in the enterovirus life cycle by facilitating RNA replication through interaction with the 2A protease.
  • Targeting SETD3 presents a potential therapeutic strategy for controlling enterovirus infections.