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Updated: Jan 19, 2026

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
Published on: August 1, 2022
The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue
Fabian F Voigt1,2, Daniel Kirschenbaum3, Evgenia Platonova4
1Brain Research Institute, University of Zurich, Zurich, Switzerland. voigt@hifo.uzh.ch.
Abstract:
Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).
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