Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nested-PCR to Detect a Specific Viral Genomic Sequence03:20

Nested-PCR to Detect a Specific Viral Genomic Sequence

1.1K
This video describes nested polymerase chain reaction, a technique that consists of two sequential PCR amplification processes using two primer sets. The first set of primers is intended to anneal to sequences upstream of the second set, resulting in selective amplification of specific gene sequences. This PCR is more sensitive and specific than a normal PCR and is widely used as a detection technique for various...
1.1K
Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology10:26

Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology

6.9K
Here, we present a rapid and cost-effective workflow for characterizing rabies virus (RABV) genomes using nanopore technology. The workflow is intended to support genomics-informed surveillance at a local level, providing information on circulating RABV lineages and their placement within regional phylogenies to guide rabies control...
6.9K
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

9.1K
Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of...
9.1K
High-Throughput Analysis of Optical Mapping Data Using ElectroMap07:36

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

10.0K
This protocol describes the setup and use of ElectroMap, a MATLAB-based open-source software platform for analysis of cardiac optical mapping data. ElectroMap provides a versatile high-throughput tool for analysis of optical mapping voltage and calcium datasets across a wide range of cardiac experimental...
10.0K
Introductory Analysis and Validation of CUT&RUN Sequencing Data04:58

Introductory Analysis and Validation of CUT&RUN Sequencing Data

4.0K
This protocol guides bioinformatics beginners through an introductory CUT&RUN analysis pipeline that enables users to complete an initial analysis and validation of CUT&RUN sequencing data. Completing the analysis steps described here, combined with downstream peak annotation, will allow users to draw mechanistic insights into chromatin...
4.0K
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy09:20

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy

14.0K
This study describes a method that allows the rapid and clear diagnosis of plant virus diseases in about half a day by using a combination of microwave assisted plant sample preparation for transmission electron microscopy and negative staining...
14.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Respiratory viral detection in adult severe acute respiratory infection post-COVID-19 pandemic: implications for antimicrobial stewardship.

The Journal of infection·2026
Same author

Andes Virus on a Cruise Ship, what it Tells us About the Global Pandemic Preparedness Agenda.

The Lancet regional health. Europe·2026
Same author

Evaluating vectors for the design of a spillover-disrupting Lassa virus transmissible vaccine.

PLoS computational biology·2026
Same author

Preparing for systematic tuberculosis screening of children and young people in a low-incidence setting: who should we target?

Archives of disease in childhood·2026
Same author

Alternative splicing broadens antiviral diversity at the human OAS2 locus.

The EMBO journal·2026
Same author

Dermcidin has antiviral activity and protects against influenza.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

6.9K

DisCVR: Rapid viral diagnosis from high-throughput sequencing data.

Maha Maabar1, Andrew J Davison1, Matej Vučak1

  • 1MRC-University of Glasgow Centre for Virus Research, Sir Michael Stoker Building, 464 Bearsden Road, Glasgow G61 1QH, UK.

Virus Evolution
|September 19, 2019
PubMed
Summary

DisCVR is a user-friendly tool for detecting human viruses using high-throughput sequencing (HTS) data. It offers high sensitivity and specificity, making HTS more accessible for clinical diagnostics and epidemiology.

Keywords:
diagnosishigh-throughput sequencingk-mervirus

More Related Videos

Nested-PCR to Detect a Specific Viral Genomic Sequence
03:20

Nested-PCR to Detect a Specific Viral Genomic Sequence

1.1K
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.1K

Related Experiment Videos

Last Updated: Jan 19, 2026

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

6.9K
Nested-PCR to Detect a Specific Viral Genomic Sequence
03:20

Nested-PCR to Detect a Specific Viral Genomic Sequence

1.1K
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

9.1K

Area of Science:

  • Virology
  • Bioinformatics
  • Genomics

Background:

  • High-throughput sequencing (HTS) offers powerful diagnostic and epidemiological potential for pathogen detection.
  • Computational and bioinformatic demands currently limit HTS application in clinical diagnostic laboratories.

Purpose of the Study:

  • To develop and validate DisCVR, a user-friendly tool for detecting human viruses from HTS data.
  • To address the computational limitations of HTS for clinical virology applications.

Main Methods:

  • DisCVR matches sample k-mers (22-nucleotide sequences) to a database of viral genome k-mers.
  • Validation involved analyzing published HTS data from 89 clinical samples of upper respiratory tract infections.
  • Comparison with real-time polymerase chain reaction (PCR) and existing metagenomic analyses.

Main Results:

  • DisCVR achieved 79% sensitivity and 100% specificity in detecting human viruses.
  • The tool successfully identified mixed viral infections.
  • Performance was comparable to established metagenomic analysis methods.

Conclusions:

  • DisCVR provides a sensitive and specific method for human virus detection from HTS data.
  • The tool is designed for accessibility, requiring limited computational resources and featuring a graphical user interface.
  • DisCVR facilitates the broader adoption of HTS in clinical diagnostics and public health surveillance.