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Related Concept Videos

The Nucleus01:32

The Nucleus

100.6K
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
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The Nucleus01:25

The Nucleus

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The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
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Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons10:13

Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons

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Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal...
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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor

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Here we present a luciferase-based biosensor to quantify the kinase activity of large tumor suppressor (LATS)-a central kinase in the Hippo signaling pathway. This biosensor has diverse applications in basic and translational research aimed at investigating Hippo pathway regulators in vitro and in...
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Actin Treadmilling01:18

Actin Treadmilling

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Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...
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Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin07:53

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

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Proteins bind to filamentous actin (F-actin) through distinct actin binding modules. In this video we demonstrate the procedure of actin co-sedimentation, which is an in vitro assay routinely used to analyze proteins or specific domains that bind...
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Related Experiment Video

Updated: Jan 19, 2026

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
10:13

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons

Published on: February 10, 2016

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When Hippo meets actin in the nucleus.

Maria Chatzifrangkeskou1, Eric O'Neill1

  • 1Department of Oncology, University of Oxford, Oxford, UK.

Molecular & Cellular Oncology
|September 19, 2019
PubMed
Summary

Nuclear actin export relies on Exportin-6 (XPO6) and RAN GTPase. RASSF1A and MST2 regulate this process, and their loss in cancer promotes tumorigenesis via nuclear actin.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Nuclear actin dynamics are crucial for cellular processes.
  • Exportin-6 (XPO6) and RAN GTPase mediate nuclear actin export.
  • The role of RASSF1A and hippo signaling in this process is not fully understood.

Purpose of the Study:

  • To investigate the role of RASSF1A and MST2 in regulating nuclear actin export.
  • To elucidate the mechanism by which RASSF1A and hippo signaling influence XPO6-RAN GTPase interaction.
  • To determine the contribution of nuclear actin to tumorigenesis in the context of RASSF1A and hippo pathway loss.

Main Methods:

  • Biochemical assays to study protein-protein interactions.
  • Cellular fractionation and imaging to track actin localization.
Keywords:
Exportin-6MRTF-ANuclear actinRASSF1Acancer

More Related Videos

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

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The Nucleus and The Nuclear Envelope
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The Nucleus and The Nuclear Envelope

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Related Experiment Videos

Last Updated: Jan 19, 2026

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
10:13

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons

Published on: February 10, 2016

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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

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The Nucleus and The Nuclear Envelope
01:32

The Nucleus and The Nuclear Envelope

100.6K
  • Genetic manipulation to assess the impact of RASSF1A and MST2 loss.

    • Main Results:

    • RASSF1A and MST2 regulate the association between XPO6 and RAN GTPase.
    • Loss of RASSF1A and hippo signaling alters nucleocytoplasmic shuttling of actin.
    • Nuclear actin accumulation in cancer cells lacking RASSF1A and hippo signaling promotes tumorigenesis.

    Conclusions:

    • RASSF1A and MST2 are key regulators of nuclear actin export.
    • Dysregulation of this pathway contributes to cancer development.
    • Targeting nuclear actin export may offer new therapeutic strategies for cancer.