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Related Concept Videos

Compact Quantum Dots for Single-molecule Imaging17:14

Compact Quantum Dots for Single-molecule Imaging

18.7K
We describe the preparation of colloidal quantum dots with minimized hydrodynamic size for single-molecule fluorescence imaging. Compared to conventional quantum dots, these nanoparticles are similar in size to globular proteins and are optimized for single-molecule brightness, stability against photodegradation, and resistance to nonspecific binding to proteins and...
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Production and Targeting of Monovalent Quantum Dots10:16

Production and Targeting of Monovalent Quantum Dots

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We provide detailed instructions for the preparation of monovalent targeted quantum dots (mQDs) from phosphorothioate DNA of defined length. DNA wrapping occurs in high yield, and therefore, products do not require purification. We demonstrate the use of the SNAP tag to target mQDs to cell-surface receptors for live-cell imaging applications.
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Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads07:13

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

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Here, we describe a protocol for the preparation of quantum dot nanobeads (QDNB) and the detection of disease biomarkers using QDNB-based lateral flow immunoassay strips. The test results can be qualitatively assessed under UV light illumination and quantitatively measured using a fluorescent strip reader within 15...
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

9.3K
We demonstrate the use of fluorescence photo activation localization microscopy (FPALM) to simultaneously image multiple types of fluorescently labeled molecules within cells. The techniques described yield the localization of thousands to hundreds of thousands of individual fluorescent labeled proteins, with a precision of tens of nanometers within single...
9.3K
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

15.7K
Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.
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Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

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Resonant excitation of a single self-assembled quantum dot can be achieved using an excitation mode orthogonal to the fluorescence collection mode. We demonstrate a method using the waveguide and Fabry-Perot modes of a planar microcavity surrounding the quantum dots. The method allows complete freedom in the detection...
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Related Experiment Video

Updated: Jan 19, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

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Quantum Dot-Based Simultaneous Multicolor Imaging.

Wenxia Wang1,2, Zhen Liu1,2, Xiaoli Lan3,4

  • 1Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Molecular Imaging and Biology
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

Quantum dots offer advanced multicolor imaging for biological research. Their tunable fluorescence enables simultaneous visualization of multiple biomarkers, aiding disease understanding and clinical decisions.

Keywords:
BiomarkersImagingMolecular imagingMulticolorQuantum dots

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Production and Targeting of Monovalent Quantum Dots
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Production and Targeting of Monovalent Quantum Dots
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Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
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Area of Science:

  • Biotechnology
  • Optical Imaging
  • Nanotechnology

Background:

  • Quantum dots (QDs) possess unique physicochemical properties valuable for optical imaging.
  • Their tunable fluorescence emission, based on size, allows excitation by a single light source.

Purpose of the Study:

  • To highlight the role of quantum dots in multicolor imaging.
  • To explore their application in understanding biomolecular interactions and disease.

Main Methods:

  • Utilizing quantum dots for simultaneous multicolor imaging of tissues and cells.
  • Real-time in situ observation of multiple biomarkers.

Main Results:

  • Quantum dots enable unmatched multitarget simultaneous multicolor imaging compared to traditional methods.
  • Facilitates quantification of biomarker expression levels.

Conclusions:

  • Quantum dot technology enhances understanding of biomolecular interactions and disease.
  • Potential applications in clinical diagnosis and treatment decision-making.