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Updated: Jan 19, 2026

Prostaglandin Extraction and Analysis in Caenorhabditis elegans
Published on: June 25, 2013
15-Hydroperoxy-PGE2 : Intermediate in Mammalian and Algal Prostaglandin Biosynthesis
Hans Jagusch1, Markus Werner2, Oliver Werz2
1Institute for Inorganic and Analytical Chemistry, Department of Instrumental Analytics/Bioorganic Analytics, Friedrich Schiller University Jena, Lessingstraße 8, 07743, Jena, Germany.
Abstract:
Arachidonic-acid-derived prostaglandins (PGs), specifically PGE2 , play a central role in inflammation and numerous immunological reactions. The enzymes of PGE2 biosynthesis are important pharmacological targets for anti-inflammatory drugs. Besides mammals, certain edible marine algae possess a comprehensive repertoire of bioactive arachidonic-acid-derived oxylipins including PGs that may account for food poisoning. Described here is the analysis of PGE2 biosynthesis in the red macroalga Gracilaria vermiculophylla that led to the identification of 15-hydroperoxy-PGE2 , a novel precursor of PGE2 and 15-keto-PGE2 . Interestingly, this novel precursor is also produced in human macrophages where it represents a key metabolite in an alternative biosynthetic PGE2 pathway in addition to the well-established arachidonic acid-PGG2 -PGH2 -PGE2 route. This alternative pathway of mammalian PGE2 biosynthesis may open novel opportunities to intervene with inflammation-related diseases.
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