Jove
Visualize
Contact Us

Related Concept Videos

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution05:08

A Practical Guide to Phage- and Robotics-Assisted Near-Continuous Evolution

2.2K
Phage- and Robotics-assisted Near-continuous Evolution (PRANCE) is a technique for rapid, robust protein evolution. Robotics allows the parallelization of experiments, real-time monitoring, and feedback...
2.2K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

35.0K
Phage display is a powerful technique to capture proteins or protein moieties that interact with an immobilized molecule of interest. Once a decision of the type of phage cDNA library to create and screen has been made, the protocol described here permits efficient affinity selection leading to identification of...
35.0K
Using Phage Display to Select Proteins with High Affinity to a Target Protein05:22

Using Phage Display to Select Proteins with High Affinity to a Target Protein

2.0K
In this video, we demonstrate the phage display technique to isolate pre-engineered phages expressing proteins with high affinity to the target...
2.0K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

19.2K
A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard...
19.2K
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

3.6K
Ubiquitination is a critical protein post-translational modification, dysregulation of which has been implicated in numerous human diseases. This protocol details how phage display can be utilized to isolate novel ubiquitin variants that can bind and modulate the activity of E3 ligases that control the specificity, efficiency, and patterns of...
3.6K
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity12:31

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

14.7K
This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in Phage Display-A Perspective.

Cold Spring Harbor protocols·2025
Same author

Erratum: Principles of Affinity Selection.

Cold Spring Harbor protocols·2024
Same author

Principles of Affinity Selection.

Cold Spring Harbor protocols·2023
Same author

Expression of a Peptidoglycan Hydrolase from Lytic Bacteriophages Atu_ph02 and Atu_ph03 Triggers Lysis of Agrobacterium tumefaciens.

Applied and environmental microbiology·2017
Same author

Refractory pain, existential suffering, and palliative care: releasing an unbearable lightness of being.

Cornell journal of law and public policy·2014
Same author

In vivo bacteriophage peptide display to tailor pharmacokinetics of biological nanoparticles.

Molecular imaging and biology·2014
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation
05:08

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Published on: January 12, 2024

2.2K

Phage Display: Simple Evolution in a Petri Dish (Nobel Lecture).

George P Smith1

  • 1University of Missouri, Division of Biological Sciences, Tucker Hall, Columbia, MO, 65211-7400, USA.

Angewandte Chemie (International Ed. in English)
|September 19, 2019
PubMed
Summary

George P. Smith recounts the development of phage-display technology, a Nobel Prize-winning technique. This scientific advance highlights gradual evolution through global research collaboration.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Phage-display technology is a powerful molecular tool.
  • Its development involved contributions from multiple researchers globally.
Keywords:
Nobel lectureaffinity selectionpeptide libraryphage display

More Related Videos

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

35.0K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.2K

Related Experiment Videos

Last Updated: Jan 19, 2026

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation
05:08

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Published on: January 12, 2024

2.2K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

35.0K
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.2K
  • Understanding its origins provides insights into scientific progress.