Related Concept Videos

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

The goal of this protocol is to show how to use Lattice Light-Sheet Microscopy to four-dimensionally visualize surface receptor dynamics in live cells. Here T cell receptors on CD4+ primary T cells are...
8.5K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand...
15.3K
Types of Receptors: Cell Surface Receptors01:28

Types of Receptors: Cell Surface Receptors

Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
26.8K
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells04:55

Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells

Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or...
11.3K
Establishing a Three-Dimensional Coculture Module of Epithelial Cells Using Nanofibrous Membranes10:08

Establishing a Three-Dimensional Coculture Module of Epithelial Cells Using Nanofibrous Membranes

Here, we demonstrate how epithelial cells cultured with fibroblasts in the nanofibrous membrane-based two-layer system can stably adhere to and grow on the membrane.
783
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Antibodies that bind to target receptors on the cell surface can confer conformation and clustering alterations. These dynamic changes have implications for characterizing drug development in target cells. This protocol utilizes confocal microscopy and image correlation spectroscopy through ImageJ/FIJI to quantify the extent of receptor clustering on the cell...
7.5K