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A simple and high-throughput method for xylem sap collection.

Manman Kan1, Kiyoshi Yamazaki1, Toru Fujiwara1

  • 1Department of Applied Biological Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, Tokyo, 113-8657, Japan.

Biotechniques
|September 19, 2019
PubMed
Summary

We developed a simple, high-throughput method for collecting xylem sap, enabling efficient analysis of water, nutrient, and signaling molecule transport. This technique effectively measures cadmium concentrations in plant xylem sap.

Keywords:
ionometranslocationxylemxylem sap

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Environmental Science

Background:

  • Xylem sap is crucial for root-to-shoot transport of water, nutrients, and signaling molecules.
  • Existing xylem sap collection methods are often low-throughput and require specialized equipment.

Purpose of the Study:

  • To develop a simple and high-throughput method for collecting small and medium volumes of xylem sap.
  • To validate the method's effectiveness in analyzing xylem sap composition, specifically cadmium concentrations.

Main Methods:

  • Development of a novel, user-friendly xylem sap collection technique.
  • Application of the method to collect both small (<200 μl) and medium (<1 ml) sample volumes.
  • Utilizing a rice cadmium transporter mutant to test the method's analytical capabilities.

Main Results:

  • The new method allows for significantly higher throughput collection of xylem sap.
  • Demonstrated successful collection of both small and medium volume xylem sap samples.
  • Effectively quantified cadmium concentrations in xylem sap using the developed method.

Conclusions:

  • The developed method offers a practical and efficient approach for xylem sap collection.
  • This technique facilitates advanced research into plant transport processes and environmental contaminant uptake.
  • Enables high-throughput analysis of xylem sap, aiding in plant physiology and toxicology studies.