Related Concept Videos

Quantitative Measurement of the Immune Response and Sleep in Drosophila12:16

Quantitative Measurement of the Immune Response and Sleep in Drosophila

To understand a link between the immune response and behavior, we describe a method to measure locomotor behavior in Drosophila during bacterial infection as well as the ability of flies to mount an immune response by monitoring survival, bacterial load, and real-time activity of a key regulator of innate immunity,...
15.9K
The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila06:06

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila

Sleep deprivation is a powerful tool to investigate sleep function and regulation. We describe a protocol to sleep deprive Drosophila using the Sleep Nullifying Apparatus, and to determine the extent of rebound sleep induced by...
4.2K
Polygraphic Recording Procedure for Measuring Sleep in Mice08:45

Polygraphic Recording Procedure for Measuring Sleep in Mice

The recording of electroencephalogram (EEG) and electromyogram (EMG) in freely behaving mice is a critical step to correlate behavior and physiology with sleep and wakefulness. The experimental protocol described herein provides a cable-based system for acquiring EEG and EMG recordings in...
25.2K
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice08:58

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Here, we describe methods of optogenetic manipulation of particular types of neurons during monitoring of sleep/wakefulness states in mice, presenting our recent work on the bed nucleus of the stria terminalis as an...
10.4K
Through-the-Wall Blood Sampling Method to Minimize Sleep Disruption in Clinical Settings06:39

Through-the-Wall Blood Sampling Method to Minimize Sleep Disruption in Clinical Settings

Here, we present a through-the-wall, minimally invasive method to sample blood during sleep in clinical...
438
Establishing a Device for Sleep Deprivation in Mice05:05

Establishing a Device for Sleep Deprivation in Mice

The present protocol outlines a method for setting up a cost-effective rocker platform-based device used for inducing sleep deprivation in mice. This device has proven to be effective in causing disruptions in electroencephalogram (EEG)-evidenced sleep patterns, as well as inducing metabolic and molecular changes associated with sleep...
2.4K