Spontaneous resolution of frontotemporal brain sagging syndrome

A spontaneous process is one that occurs naturally under certain conditions. A nonspontaneous process, on the other hand, will not take place unless it is “driven” by the continual input of energy from an external source. Processes have a natural tendency to occur in one direction under a given set of conditions. Water will naturally flow downhill (spontaneous process), but uphill flow (nonspontaneous process) requires outside intervention such as the use of a pump. Iron exposed to...

This protocol describes the preparation of horizontal hippocampal-entorhinal cortex (HEC) slices from mice exhibiting spontaneous sharp-wave ripple activity. Slices are incubated in a simplified interface holding chamber and recordings are performed under submerged conditions with fast-flowing artificial cerebrospinal fluid to promote tissue oxygenation and the spontaneous emergence of network-level...

In this study, the authors report for the first time a novel 3D-Immersive & Interactive Neuronavigation (3D-IIN) through the impact of a spontaneous migraine headache attack in the μ-opioid system of a patient's brain in...

This video demonstrates the induction of ictal events in coronal cortical slices from a transgenic mouse expressing light-activated cation channels in cortical excitatory pyramidal neurons. The slice is placed in a recording chamber, and a recording electrode is inserted into the superficial cortical layer. Upon perfusion with a seizure-inducing drug that blocks potassium channels, the neurons become hyperexcitable and generate spontaneous ictal events. To generate on-demand ictal events, a...

Here, we present a protocol that uses near-infrared dyes in conjunction with immunohistochemistry and high-resolution scanning to assay proteins in brain...

Source: Villaseñor, R and Collin. L. High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis. J. Vis. Exp. (2017)This video demonstrates high-resolution 3D imaging of the blood-brain barrier (BBB) using confocal microscopy, showing antibody-stained mouse brain sections to analyze BBB components and intracellular structures, applicable for studying brain homeostasis, neurovascular interactions, and disease-related BBB...