Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

110.6K
Overview
110.6K
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

7.6K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
7.6K
Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

80.9K
Clathrin-mediated endocytosis, a rapid and highly dynamic process internalizes many proteins, including signaling receptors. The protocol described here directly visualizes the kinetics of individual endocytic events. This is essential for understanding how core members of the endocytic machinery coordinate with each other, and how protein cargo influence this process.
80.9K
In vivo and in vitro Studies of Adaptor-clathrin Interaction17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

15.0K
Clathrin-mediated endocytosis depends on adaptor proteins that coordinate cargo selection and clathrin coat assembly. Here we describe procedures to study adaptor-clathrin physical interaction and live cell imaging approaches using as a model the yeast endocytic adaptor protein...
15.0K
An Introduction to Endocytosis and Exocytosis09:27

An Introduction to Endocytosis and Exocytosis

79.5K
Cells can take in substances from the extracellular environment by endocytosis and actively release molecules into it by exocytosis. Such processes involve lipid membrane-bound sacs called vesicles. Knowledge of the molecular architecture and mechanisms of both is key to understanding normal cell physiology, as well as the disease states that arise when they become defective. This video will first briefly review a few pivotal discoveries in the history of endo- and exocytosis research. Next,...
79.5K
Endocytosis01:16

Endocytosis

12.3K
Eukaryotic cells acquire nutrients for growth and proliferation. Nutrients and other molecules that require degradation are internalized from the extracellular space by a process called endocytosis. The term ‘endocytosis' was first coined by Christian de Duve in 1963.
Endocytosis always begins with the plasma membrane enclosing an incoming molecule to form a transport vesicle which, in some cases, can be coated with a protein called ‘clathrin.' Endocytosed material is either...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward a PSMA PET-mpMRI pathway for biopsy decision-making in men with suspected prostate cancer: interim results from the prospective BIOPSTAGE trial.

Frontiers in oncology·2026
Same author

How Regulation 536/2014 Is Changing Academic Research with Therapeutic Radiopharmaceuticals: A Local Experience.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Efficacy, toxicity, and clinical outcomes of 177Lu-PSMA-617 radioligand therapy at 5.5 GBq per cycle in patients with advanced castration-resistant prostate cancer: a prospective, single arm, phase II study.

European journal of nuclear medicine and molecular imaging·2025
Same author

Production and Quality Control of [<sup>68</sup>Ga]Ga-FAPI-46: Development of an Investigational Medicinal Product Dossier for a Bicentric Clinical Trial.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

From Biological Waste to Therapeutic Resources: A Comprehensive Review of Stem Cell Sources, Characterization, and Biomedical Potentials.

Stem cell reviews and reports·2025
Same author

Hyaluronic Acid-Decorated Liposomes for the Intrapulmonary Delivery of Imatinib: A Targeted Treatment for Postinflammatory Pulmonary Fibrosis.

Small science·2025

Related Experiment Video

Updated: Jan 19, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

80.9K

Clathrin mediated endocytosis - Methods and Protocols.

Manuela Monti1

  • 1Biotechnology laboratories, Research Center for Regenerative Medicine, Fondazione IRCCS Policlinico San Matteo, Pavia. M.Monti@smatteo.pv.it.

European Journal of Histochemistry : EJH
|September 19, 2019
PubMed
Summary

Clathrin, a moonlighting protein, regulates vesicular trafficking and is key to Clathrin-Mediated Endocytosis (CME). This protein performs multiple biochemical and biophysical functions critical for cellular processes.

More Related Videos

Clathrin and Receptor-mediated Endocytosis
01:39

Clathrin and Receptor-mediated Endocytosis

110.6K
Receptor-mediated Endocytosis
01:20

Receptor-mediated Endocytosis

7.6K

Related Experiment Videos

Last Updated: Jan 19, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

80.9K
Clathrin and Receptor-mediated Endocytosis
01:39

Clathrin and Receptor-mediated Endocytosis

110.6K
Receptor-mediated Endocytosis
01:20

Receptor-mediated Endocytosis

7.6K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biophysics

Background:

  • Clathrin is a 'moonlighting protein' with diverse biochemical and biophysical roles.
  • It is the primary component regulating vesicular trafficking via Clathrin-Mediated Endocytosis (CME).

Purpose of the Study:

  • To explore the multifaceted functions of clathrin beyond its canonical role in endocytosis.
  • To elucidate the 'moonlighting' aspects of clathrin in cellular processes.

Main Methods:

  • Literature review of biochemical and biophysical studies on clathrin.
  • Analysis of clathrin's involvement in various cellular pathways.

Main Results:

  • Clathrin's involvement in processes beyond endocytosis, such as membrane remodeling and signal transduction.
  • Evidence supporting its role as a master regulator in multiple cellular functions.

Conclusions:

  • Clathrin's 'moonlighting' nature highlights its significance in cellular regulation.
  • Understanding these diverse functions is crucial for comprehending cellular mechanics and disease.