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Strategy for achieving standardized bone models.

Mikhael Hadida1, David Marchat1

  • 1Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, Saint-Etienne, France.

Biotechnology and Bioengineering
|September 19, 2019
PubMed
Summary

Developing functional three-dimensional bone tissue models is challenging. This review explores perfusion flow control as a key factor for creating advanced organ-on-chip systems for better biology research and drug development.

Keywords:
bonein vitro 3D modelsperfusion bioreactortissue engineering

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Organ-on-chip systems offer advanced in vitro models for research and drug development.
  • Current three-dimensional (3D) bone tissue models are limited in functionality.
  • Significant progress has been made in organ-on-chip technology, but 3D bone models remain elusive.

Purpose of the Study:

  • To investigate the role of perfusion flow control in developing functional 3D bone tissue models.
  • To identify perfusion flow effects as a critical missing link for advanced bone models.
  • To propose a roadmap for creating scientifically exploitable 3D bone models.

Main Methods:

  • Review of current literature on organ-on-chip systems and bone tissue engineering.
  • Analysis of the impact of perfusion flow on cellular behavior and tissue development in vitro.
  • Identification of key parameters for controlling perfusion in 3D culture systems.

Main Results:

  • Perfusion flow significantly influences nutrient/waste exchange and cellular organization in engineered tissues.
  • Controlled perfusion is essential for mimicking native bone microenvironments.
  • Lack of controlled perfusion hinders the development of functional 3D bone models.

Conclusions:

  • Optimizing perfusion flow is critical for advancing 3D bone tissue engineering.
  • Implementing controlled perfusion in organ-on-chip systems will enable the creation of functional in vitro bone models.
  • This research provides a roadmap towards improved bone modeling for drug discovery and biological studies.