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Mouse kidney androgen-regulated protein messenger ribonucleic acid is expressed in the proximal convoluted tubules

A Meseguer1, J F Catterall

  • 1Population Council, Rockefeller University, New York, New York 10021.

Molecular Endocrinology (Baltimore, Md.)
|August 1, 1987
PubMed

Insights

Kidney androgen-regulated protein (KAP) mRNA is highly abundant in mouse kidneys. Androgens induce KAP mRNA in specific kidney tubule cells, with distinct responses in different regions after hormone treatment.

Area of Science:

  • Molecular Endocrinology
  • Renal Physiology
  • Gene Regulation

Background:

  • Kidney androgen-regulated protein (KAP) mRNA is a highly abundant species in mouse kidney.
  • Androgens differentially regulate various mRNAs in the kidney.
  • KAP mRNA shows unusual sensitivity to low androgen-receptor complex concentrations.

Purpose of the Study:

  • To identify the specific kidney cells responsible for KAP mRNA synthesis.
  • To understand the cellular response of KAP mRNA production to androgens.

Main Methods:

  • In situ hybridization using single-stranded RNA probes.
  • Analysis of androgen-regulated gene expression in mouse kidney.

Main Results:

  • KAP mRNA is synthesized in the epithelial cells of renal proximal convoluted tubules.
  • In castrated males, KAP mRNA is found in a subpopulation of juxtamedullary cortical tubule cells.
  • Androgen treatment induces KAP mRNA expression in cortical proximal tubules.

Conclusions:

  • Two distinct androgen responses are observed in kidney tubule cells.
  • One response involves increased KAP gene expression in juxtamedullary cells.
  • The other response involves the recruitment of cortical tubule cells for KAP mRNA synthesis.

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