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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Updated: Jan 19, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches.

Wenjing Liu¹, Yourong Guo², Kaizhe Wang¹

¹Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, China.

|September 20, 2019

Summary

Addressable DNA origami enables high-throughput single-molecule force spectroscopy. This method clearly differentiates DNA targets with single base-pair mismatches using precise force measurements.

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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Area of Science:

Biophysics
Molecular Biology
Nanotechnology

Background:

Single-molecule force spectroscopy (SMFS) using atomic force microscopy (AFM) is crucial for understanding molecular interactions.
A major limitation of current AFM-based SMFS is its low throughput, hindering comprehensive molecular studies.

Purpose of the Study:

To develop a novel method for high-throughput analysis of multiple target molecules using SMFS.
To demonstrate the capability of the new method in differentiating subtle molecular variations.

Main Methods:

Utilized addressable DNA origami nanostructures to create arrays of specific target molecules.
Employed atomic force microscopy-based single-molecule-force spectroscopy to probe molecular interactions and measure rupture forces.

Main Results:

Successfully studied multiple target molecules simultaneously, significantly increasing throughput compared to conventional methods.
Clearly differentiated six target DNA molecules that varied by only a single base-pair mismatch.
Achieved precise differentiation at a low rupture force of approximately 4 pN.

Conclusions:

Addressable DNA origami provides a powerful platform for enhancing the throughput of single-molecule force spectroscopy.
This technique enables sensitive detection of single base-pair differences in DNA molecules.
The method holds promise for applications in molecular diagnostics and fundamental biological research.