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Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches
Wenjing Liu1, Yourong Guo2, Kaizhe Wang1
1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. hujun@sinap.ac.cn libin@sinap.ac.cn and University of Chinese Academy of Sciences, Beijing 100049, China.
Nanoscale
|September 20, 2019
Abstract:
Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.

